Development and clinical application of a novel method for the detection of circulating tumor cells

• Project/Area Number: 15H04929
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Konno Hiroyuki 浜松医科大学, 医学部, 学長 (00138033)
• Co-Investigator(Kenkyū-buntansha): 平松 良浩 浜松医科大学, 医学部, 特任准教授 (00397390) ; 神谷 欣志 浜松医科大学, 医学部附属病院, 講師 (20324361) ; 菊池 寛利 浜松医科大学, 医学部附属病院, 講師 (70397389)
• Research Collaborator: Ozaki Yusuke 浜松医科大学, 医学部附属病院, 診療助教 ; Hirotsu Amane 浜松医科大学, 大学院生
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000); Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
• Keywords: 循環腫瘍細胞 / 非染色 / 蛍光位相顕微鏡 / 消化器癌 / 血中循環腫瘍細胞 / 転移 / 定量位相顕微鏡 / 機械学習 / 癌 / 細胞・組織

Outline of Final Research Achievements

Circulating tumor cells (CTCs) are used as a liquid biopsy target in cancer patients. In this study, we sought to develop a novel CTC detection method using quantitative phase microscopy (QPM) and computer vision technologies for pattern recognition based on the morphological features of live cells. We observed white blood cells (WBCs) and cancer cell line cells by QPM without staining, and successfully differentiated WBCs from cell lines. We applied our image recognition methods to the cells flowing in the chamber, and properly differentiated WBCs from cell line cells flowing in the chamber. Finally, cell fractions suspicious of CTCs were detected in blood samples of tumor bearing mice and cancer patients.

Research Products

• [Presentation] 定量位相顕微鏡と画像認証システムを用いた新たな血中循環腫瘍細胞の検出法 (2018)
  • Author(s): 廣津周、菊池寛利、尾﨑裕介、川田三四郎、村上智洋、松本知拓、川端俊貴、平松良浩、神谷欣志、坂口孝宣、山田秀直、岡崎茂俊、今野弘之、竹内裕也
  • Organizer: 第118回日本外科学会定期学術集会
• [Presentation] Label-free classification of live cells using quantitative phase microscopy images for negative selection of circulating tumor cells (2018)
  • Author(s): Hirotoshi Kikuchi, Yusuke Ozaki, Amane Hirotsu, Hidenao Yamada, Shigetoshi Okazaki, Tomohiro Murakami, Tomohiro Matsumoto, Yoshihiro Hiramatsu, Kinji Kamiya, Takanori Sakaguchi, Hiroyuki Konno, Hiroya Takauchi
  • Organizer: American Association for Cancer Research Annual Meeting 2018
  • Int'l Joint Research
• [Presentation] 定量位相顕微鏡を用いた新たな血中循環腫瘍細胞の検出法 (2018)
  • Author(s): 廣津周、菊池寛利、尾﨑裕介、山田秀直、岡崎茂俊、平松良浩、神谷欣志、坂口孝宣、今野弘之、竹内裕也
  • Organizer: 第73回日本消化器外科学会総会
• [Presentation] Label free imaging identification of white blood cells using quantitative phase microscope for negative selection of circulating tumor cells (2017)
  • Author(s): Yusuke Ozaki, Amane Hirotsu, Hidenao Yamada, Hirotoshi Kikuchi, Tomohiro Murakami, Tomohiro Matsumoto, Yoshihiro Hiramatsu, Kinji Kamiya, Kentaro Goto, Toyohiko Yamauchi, Yukio Ueda, Shigetoshi Okazaki, Hiroyuki Konno , Hiroya Takeuchi
  • Organizer: 3rd Advances in Circulating Tumour Cells
  • Int'l Joint Research
• [Presentation] Label-free imaging identification of cells using quantitative phase microscopy for negative selection of Circulating Tumor Cells (2016)
  • Author(s): Yusuke Ozaki，Hidenao Yamada，Hirotoshi Kikuchi，Tomohiro Matsumoto，Toshiki Kawabata，Yoshihiro Hiramatsu，Manabu Ohta，Kinji Kamiya，Takanori Sakaguch，Shigetoshi Okazaki，Hiroyuki Konno
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: 京都府京都市
  • Year and Date: 2016-10-23
  • Int'l Joint Research
• [Presentation] 定量位相顕微鏡と画像認証システムを用いた新たな血中循環腫瘍細胞検出手法の試み (2016)
  • Author(s): 尾﨑裕介、山田秀直、菊池寛利、廣津周、村上智洋、松本知拓、川端俊貴、平松良浩、太田学、神谷欣志、坂口孝宣、今野弘之
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2016-10-06
• [Presentation] 定量位相顕微鏡と画像認証システムを利用した新たな血中循環腫瘍細胞検出の試み (2016)
  • Author(s): 尾﨑裕介、山田秀直、菊池寛利、川端俊貴、平松良浩、太田学、神谷欣志、坂口孝宣、岡崎茂俊、今野弘之
  • Organizer: 第71回日本消化器外科学会総会
  • Place of Presentation: 徳島県徳島市
  • Year and Date: 2016-07-14
• [Presentation] Label-free imaging identification of WBCs based on the features of Quantitative Phase Microscope Images for negative selection of CTCs (2016)
  • Author(s): Yusuke Ozaki, Hidenao Yamada, Hirotoshi Kikuchi, Tomohiro Murakami, Tomhiro Matsumoto, Toshiki Kawabata, Yoshihiro Hiramatsu, Manabu Ohta, Kinji Kamiya, Megumi Baba, Toyohiko Yamauchi, Kentaro Goto, Yukio Ueda, Shigetoshi Okazaki, Hiroyuki Konno
  • Organizer: American Association for Cancer Research Annual Meeting 2016
  • Place of Presentation: New Orleans, LA, USA
  • Year and Date: 2016-04-19
• [Presentation] Label-free imaging identification of WBCs based on the features of Quantitative Phase Microscope Images for negative selection of CTCs (2016)
  • Author(s): Yusuke Ozaki，Hidenao Yamada，Hirotoshi Kikuchi，Tomohiro Murakami，Tomohiro Matsumoto，Toshiki Kawabata， Yoshihiro Hiramatsu，Manabu Ohta，Kinji Kamiya，Megumi Baba, Toyohiko Yamauchi, Kentaro Goto, Yukio Ueda, Shigetoshi Okazaki，Hiroyuki Konno
  • Organizer: American Association for Cancer Research Annual Meeting 2016,
  • Place of Presentation: New Orleans, LA, USA
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] 定量位相顕微鏡と画像認証システムを用いた新たな血中循環腫瘍細胞（CTC）検出手法の試み (2016)
  • Author(s): 尾﨑裕介、山田秀直、菊池寛利、村上智洋、松本知拓、高橋善明、川端俊貴、平松良浩、太田学、神谷欣志、坂口孝宣、海野直樹、岡崎茂俊、今野弘之
  • Organizer: 第116 回日本外科学会定期学術集会
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2016-04-14
• [Presentation] 定量位相顕微鏡と画像認証システムを利用した新たな血中循環腫瘍細胞検出の試み (2015)
  • Author(s): 尾崎裕介、菊池寛利、村上智洋、松本知拓、川端俊貴、平松良浩、太田学、神谷欣志、坂口孝宣、今野弘之
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2015-10-10
• [Presentation] 定量位相顕微鏡と画像認証システムを利用した新たな血中循環腫瘍細胞検出の試み (2015)
  • Author(s): 尾﨑裕介、山田秀直、菊池寛利、松本知拓、川端俊貴、平松良浩、太田学、神谷欣志、坂口孝宣、今野弘之
  • Organizer: 第70回日本消化器外科学会総会
  • Place of Presentation: 静岡県浜松市
  • Year and Date: 2015-07-15