Investigation of allogeneic synovial mesenchymal stem cells for cartilage and meniscus regeneration therapy
| Project/Area Number |
15H04953
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
SEKIYA Ichiro 東京医科歯科大学, 統合研究機構, 教授 (10345291)
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| Co-Investigator(Kenkyū-buntansha) |
辻 邦和 東京医科歯科大学, 大学院医歯学総合研究科, 寄附講座准教授 (20323694)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | 移植・再生医療 / 再生医療 |
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| Outline of Final Research Achievements |
Conflicting evidence exists regarding the immunosuppressive capacity of mesenchymal stem cells (MSCs). Our aim was to examine the effects of inducing recipient T cell responses by dead allogeneic MSCs and of suppressing the responses by living MSCs. Synovial fluid (SF) was obtained from patients who underwent knee surgeries. SF mononuclear cells were co-cultured with dead allogeneic MSCs. Immunosuppressive capacity of MSCs was verified by the addition of living MSCs to the co-culture. CD141+ dendritic cells and CD141lowCD11b+CD14+ macrophages were identified in patient SF. Dead allogeneic MSCs were taken up by synovial APCs and induced T cell proliferation and cytokine production such as granzyme B and IL-6. Addition of living MSCs counteracted T cell proliferation and granzyme B production. Dead allogeneic MSCs elicited cytotoxic T cell responses with the help of Th1 cytokines but living MSCs counteract this and direct intra-articular environment to anti-inflammatory condition.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Yields and chondrogenic potential of primary synovial mesenchymal stem cells are comparable between rheumatoid arthritis and osteoarthritis patients.2017
Author(s)
Yuji Kohno, Mitsuru Mizuno, Nobutake Ozeki, Hisako Katano, Keiichiro Komori, Shizuka Fujii, Koji Otabe, Masafumi Horie, Hideyuki Koga, Kunikazu Tsuji, Mikio Matsumoto, Haruka Kaneko, Yuji Takazawa, Takeshi Muneta, Ichiro Sekiya
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Journal Title
Stem Cell Res Ther
Volume: 8
Issue: 1
Pages: 115-115
DOI
Related Report
Peer Reviewed / Open Access
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