Development of an innovative imaging system for clarifying cell-cell interactions linking cancer bone metastasis and bone metabolism
| Project/Area Number |
15H04962
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Ehime University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | バイオテクノロジー / シグンル伝達 / 遺伝子 / タンパク質 / 細胞・組織 |
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| Outline of Final Research Achievements |
In order to visualize and analyze the intercellular interaction of cancer cells and microenvironmental cells in breast cancer bone metastases in living mouse, we developed an innovative fluorescence imaging system of multicolor imaging with high spatial resolution or high temporal resolution within the bone marrow. Furthermore, we established bone metastasis models using breast cancer cell lines expressing various fluorescent proteins, and successfully imaged cancer cells and microenvironment including blood vessels, stromal cells, matrix collagen in the bone marrow. Finally, in bone marrow, we identified breast cancer cells that are involved in resistance to anticancer drugs that survive administration of 5 FU of anticancer drugs, and succeeded in imaging the interaction with various microenvironmental cells.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM).2016
Author(s)
Yamamoto S, Oshima Y, Saitou T, Watanabe T, Miyake T, Yoshida O, Tokumoto Y, Abe M, Matsuura B, Hiasa Y, Imamura T
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Journal Title
Biochemistry and Biophysics Reports
Volume: 8
Pages: 277-283
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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