Development of revitalization therapy for stem cell depleted conditions

• Project/Area Number: 15H05002
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Jichi Medical University
• Principal Investigator: Yoshimura Kotaro 自治医科大学, 医学部, 教授 (60210762)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: 幹細胞 / 放射線障害 / 放射線 / 虚血下肢 / 糖尿病 / 再生医学

Outline of Final Research Achievements

Clinical sequelae of irradiation result in tissue devitalization, where wound healing capacity is impaired. Non-lethal irradiation at various doses (5-15 Gy) and frequencies (1-3 times on sequential days) was delivered to dorsal skin of nude mice.Wounds were locally injected with vehicle, cultured adipose-derived stem cells (ASCs), centrifuged fat tissue, or micronized cellular adipose matrix, monitoring therapeutic impact for up to 15 days. Radiation damage was dose-dependent. At highest dosage (15 Gy × 3), open wounds persisted on Day 15. However, wounds injected with cultured ASCs were nearly healed on Day 12, and those treated with injection of centrifuged fat or micronized connective tissue healed faster than untreated controls (p<0.05).Irradiation damage and tissue devitalization were dose-dependent, though fractionated protocols helped to reduce. ASCs and other fat-derived products harboring ASCs successfully revitalized irradiated tissues and accelerated wound healing.

Research Products

• [Journal Article] Therapeutic effects of human adipose-derived products on impaired wound healing in irradiated tissue (2018)
  • Author(s): Szu-Hsien Wu, Takako Shirado., Takanobu Mashiko, Jingwei Feng, Rintaro Asahi, Koji Kanayama, Masanori Mori, Daekwan Chi, Ataru Sunaga,, Shunji Sarukawa, Kotaro Yoshimura
  • Journal Title: Plast Reconstr Surg Volume: -
  • Peer Reviewed