| Project/Area Number |
15H05016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
赤池 孝章 東北大学, 医学系研究科, 教授 (20231798)
宮本 洋一 昭和大学, 歯学部, 准教授 (20295132)
山田 篤 昭和大学, 歯学部, 講師 (50407558)
片桐 岳信 埼玉医科大学, 医学部, 教授 (80245802)
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| Co-Investigator(Renkei-kenkyūsha) |
TANAKA Sakae 東京大学, 医学系研究科, 教授 (50282661)
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| Research Collaborator |
SUZUKI Dai 昭和大学, 歯学部, 講師 (00585797)
YOSHIMURA Kentaro 昭和大学, 歯学部, 助教 (10585699)
FUNATO Sakie 昭和大学, 歯学部, 助教 (20783252)
IZUMIDA Eri 昭和大学, 歯学部, 助教 (70783497)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | 軟骨細胞 / 骨芽細胞 / 破骨細胞 / 一酸化窒素 / 活性酸素種 / 8-nitro-cGMP / 骨細胞 / 骨代謝 / 8-NO2-cGMP / S-グアニル化 / 硬組織疾患 / 疾患モデルマウス / 硬組織 / 活性酸素 / はタンパク質S-グアニル / 細胞分化 / 細胞増殖 |
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| Outline of Final Research Achievements |
We investigated the production and functions of 8-nitro-cGMP, a novel second messenger of nitric oxide and reactive oxygen species, in the cartilage and bone tissues. It had been accepted that cGMP is a mediator of bone growth. In this study, we found that not only cGMP but also 8-nitro-cGMP was produced in chondrocytes in the growth plate cartilage. In addition, 8-nitro-cGMP promoted the proliferation of chondrocytes in the growth plates, which caused enhancement in bone growth. Secondly, we found that production of 8-nitro-cGMP in osteoclast precursor cells after exposure to inflammatory cytokines. Since 8-nitro-cGMP was found to promote osteoclast differentiation, it is suggested that inflammatory bone resorption is explained, at least in part, by increased production of 8-nitro-cGMP under inflammatory conditions.
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