Functional Cytokine network in the Oral Cancer Stem Cell Niche and its applicastion for diagnosis and treatment
| Project/Area Number |
15H05043
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
Okamoto Tetsuji 広島大学, 医歯薬保健学研究科(歯), 教授 (00169153)
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| Co-Investigator(Kenkyū-buntansha) |
林堂 安貴 広島大学, 病院(歯), 講師 (70243251)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | 口腔扁平上皮癌 / 癌幹細胞 / 無血清培養 / exosome / CD133 / Side population細胞 / rBC2LCN陽性細胞 / IGF-2 / 口腔扁平上皮癌細胞 / ビタミンD誘導体 / エクソソーム / micro RNA / chemical messenger / autocrine factor / 口腔がん / がん幹細胞 / sphere形成 / 分子標的治療薬 / rBC2LCN / CD133陽性細胞 / sphere形成能 / 口腔扁平上皮がん細胞 / sphere 形成細胞 / 細胞内分泌学 / レクチンマーカー / 口腔癌幹細胞 / セルソーター / フローサイトメーター / ボイデンチャンバー法 |
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| Outline of Final Research Achievements |
In this study, we aim to develop a novel diagnostic and therapeutic method targeting cancer stem cells in human oral squamous cell carcinoma (OSCC), and developed a specific carbohydrate structure expressed on the cell surface of undifferentiated pluripotent stem cells We analyzed the cell and molecular biological characteristics of rBC2 LCN-recognizing carbohydrate positive cell group isolated from human oral squamous-cell carcinoma-derived cell line using rBC2LCN which recognizes lectin. Furthermore, we clarified a niche mechanism controlling self-renewal ability and differentiation ability, clarify its cell / molecular biological characteristics through identification of cytokine network molecule / signal group necessary for maintaining niche function, We aim to establish diagnostic and therapeutic methods for new cancer targeting stem cells.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Eldecalcitol (ED-71), an analog of 1α,25(OH)2D3, inhibits the growth of squamous cell carcinoma (SCC) cells in vitro and in vivo by down-regulating expression of heparin-binding protein 17/fibroblast growth factor-binding protein-1 (HBp17/FGFBP-1) and FGF-22017
Author(s)
T. Shintani, F. Takatsu, S. N. Z. Rosli, E. Usui, Atsuko Hamada, K. Sumi, Y. Hayashido, S. Toratani, Tetsuji Okamoto
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Journal Title
In Vitro Cellular & Developmental Biology - Animal
Volume: 53
Issue: 9
Pages: 810-817
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Generation and maintenance of human induced pluripotent stem cells in serum-free and feeder-free culture condition-comparison of various target cells and virus vectors2016
Author(s)
E. Akagi, S. Yamasaki, A. Hamada, H. Nakatao, F. Obayashi, T. Yasui, Y. Taguchi, M. Ohtaka, K. Nishimura, M. Nakanishi, T. Okamoto
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Journal Title
Jpn. J. Tissue Cult. Soc. Dent. Res,
Volume: 25(1),
Pages: 21-22
Related Report
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[Journal Article] Generation of Disease-specific human iPSCs in integration-, feeder-, and serum-free culture2016
Author(s)
A. Hamada, H. Nakatao, F. Obayashi, T. Yasui, E. Akagi, S. Yamasaki, S. Toratani, T. Okamoto
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Journal Title
Jpn. J. Tissue Cult. Soc. Dent. Res
Volume: 25(1),
Pages: 23-24
Related Report
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[Journal Article] Generation of cleidocranial dysplasia-specific human induced pluripotent stem cells in completely serum-, feeder-, and integration-free culture.2015
Author(s)
Yamasaki S, Hamada A, Akagi E, Nakatao H, Ohtaka M, Nishimura K, Nakanishi M, Toratani S, Okamoto T.
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Journal Title
In Vitro Cell Dev Biol Anim.
Volume: 52
Issue: 2
Pages: 252-264
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Comparison of the prognosis of bisphosphonate-related osteonecrosis of the jaw caused by oral and intravenous bisphosphonates.2015
Author(s)
Shintani T, Hayashido Y, Mukasa H, Akagi E, Hoshino M, Ishida Y, Hamana, T. Okamoto K, Kanda T, Koizumi K, Yoshioka Y, Tani R, Toratani S, Okamoto T.
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Journal Title
Int J Oral Maxillofac Surg.
Volume: Jul;44(7):
Pages: 840-844
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Establishment and characterization of normal and disease-specific human iPSCs in serum-, integration- and feeder-free culture: Plenary Symposium on Infinite Potential of Stem Cells2016
Author(s)
S. Yamasaki, A. Hamada, H. Nakatao, E. Akagi, F. Ohbayashi, T. Yasui, S. Toratani, T. Okamoto,
Organizer
2016 World Congress on In Vitro Biology
Place of Presentation
San Diego, California, USA
Year and Date
2016-06-14
Related Report
Int'l Joint Research / Invited
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