Vaccines against periodontitis using DNA ORIGAMI and imidazoquinolines

• Project/Area Number: 15H05052
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontology
• Research Institution: Niigata University
• Principal Investigator: KOICHI TABETA 新潟大学, 医歯学系, 研究准教授 (20401763)
• Co-Investigator(Kenkyū-buntansha): 寺尾 豊 新潟大学, 医歯学系, 教授 (50397717) ; 高橋 直紀 新潟大学, 医歯学総合研究科, 特任講師 (80722842)
• Co-Investigator(Renkei-kenkyūsha): YAMAZAKI Kazuhisa 新潟大学, 医歯学系, 教授 (00182478)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: 歯周病 / ワクチン / IgA / PCSK9 / 核酸抗原 / 核酸医薬 / 唾液 / 歯学 / 免疫学 / 歯周炎

Outline of Final Research Achievements

Although the efficacy of vaccines against an infectious disease, periodontitis, has been suggested, it has not been applied in clinical practices. In this study, we examined the possibility of development of a vaccine to prevent/treat periodontitis focusing on induction of secretory IgA production via modulation of mucosal immunity. We found that, chemokine production and follicular T cell activation were induced by imidazoquinoline derivatives which are nucleic acid antigen - like compounds, and secretory IgA production was promoted. These findings suggested the possibility of developing a periodontitis vaccine using nucleic acid antigen as an adjuvant.

Research Products

• [Int'l Joint Research] テキサス大学/サウスウェスタンメディカルセンター/Bruce Beutler(米国)
• [Int'l Joint Research] テキサス州立大学サウスウエスタンメディカルセンター(米国)
• [Int'l Joint Research] テキサス大学 サウスメディカルメディカルセンター(米国)
• [Journal Article] Increased serum PCSK9, a potential biomarker to screen for periodontitis, and decreased total bilirubin associated with probing depth in a Japanese community survey (2018)
  • Author(s): Tabeta K.、Hosojima M.、Nakajima M.、Miyauchi S.、Miyazawa H.、Takahashi N.、Matsuda Y.、Sugita N.、Komatsu Y.、Sato K.、Ishikawa T.、Akiishi K.、Yamazaki K.、Kato K.、Saito A.、Yoshie H.
  • Journal Title: Journal of Periodontal Research Volume: 印刷中 Issue: 3 Pages: 446-456
  • DOI: 10.1111/jre.12533
  • Peer Reviewed / Open Access
• [Presentation] 細菌抗原によるPCSK9産生の誘導機構 (2016)
  • Author(s): 横地麻衣, 多部田康一, 宮澤春菜, 野中由香莉, 高橋直紀, 松田由実, 佐藤圭祐, 山田実生, 伊藤晴江, 中島貴子, 山崎和久
  • Organizer: 日本歯科保存学会2016年度秋季学術大会
  • Place of Presentation: キッセイ文化ホール（長野県松本市）
  • Year and Date: 2016-10-28