Development of rapid diagnosis methods based on pathogen genome and their validation using clinical sample
| Project/Area Number |
15H05272
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Hokkaido University |
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Principal Investigator |
Yamagishi Junya 北海道大学, 人獣共通感染症リサーチセンター, 准教授 (80535328)
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 穣 東京大学, 大学院新領域創成科学研究科, 教授 (40323646)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Keywords | 感染症 / ゲノム / 次世代シーケンサー / 網羅的診断 / 感染症迅速診断 / ナノポアシーケンサー / 非特異的病原体検出 / メタゲノム / MinION / diagnosis-by-sequencing / ウイルス / 原虫 / マラリア / デングウイルス / 迅速診断 / 遺伝子型 |
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| Outline of Final Research Achievements |
To develop novel system for rapid diagnosis of infectious diseases based on pathogen genome, we had attempted to integrate 1) MinION, a rapid real-time portable next generation sequencer available in the market recently, and isothermal amplification system such as 2) Loop-mediated Isothermal Amplification (LAMP) method and 3) non- or semi-specific multiple displacement amplification (MDA) method in this study. The whole system including sequencing and library preparation is totally portable; therefore, it is executable in any place in standalone. We had proved the concept of the system at the lab then successfully demonstrated that it is feasible even at clinical site including Indonesia where various infectious are endemic.
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| Academic Significance and Societal Importance of the Research Achievements |
MinIONは小型迅速であるだけでなく省電力でもあり、ノートPCのUSBから電力の供給が可能である。そこで、今回開発した乾燥LAMP法あるいはMDA法を応用した非特異的検体処理法と組み合わせることで、遺伝子解析システム一式のポータビリティーと汎用性が革新的に向上することが予想される（"どこでも・なんでもシーケンス"の実現）。これら特徴を病原体の一次診断に用いることで、途上国において遺伝子型判定に基づく先進医療の提供が可能になるだけでなく、日本を含む先進国においても感染症の国境を越えた移動とそのアウトブレイクに対する対策を提供できる。
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] An innovative diagnostic technology for the codon mutation C580Y in kelch13 of Plasmodium falciparum with MinION nanopore sequencer.2018
Author(s)
Kazuo Imai, Norihito Tarumoto, Lucky Ronald Runtuwene, Jun Sakai, Kyoko Hayashida, Yuki Eshita, Ryuichiro Maeda, Josef Tuda, Hideaki Ohno, Takashi Murakami, Shigefumi Maesaki, Yutaka Suzuki, Junya Yamagishi, Takuya Maeda.
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Journal Title
Malaria J
Volume: 29
Issue: 1
Pages: 217-217
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Serotyping dengue virus with isothermal amplification and a portable sequencer2017
Author(s)
Yamagishi Junya、Runtuwene Lucky R.、Hayashida Kyoko、Mongan Arthur E.、Thi Lan Anh Nguyen、Thuy Linh Nguyen、Nhat Cam Nguyen、Limkittikul Kriengsak、Sirivichayakul Chukiat、Sathirapongsasuti Nuankanya、Frith Martin、Makalowski Wojciech、Eshita Yuki、Sugano Sumio、Suzuki Yutaka
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Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 3510-3510
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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