Development of a malaria hot-spot surveillance system

• Project/Area Number: 15H05276
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Ehime University
• Principal Investigator: Tsuboi Takafumi 愛媛大学, プロテオサイエンスセンター, 教授 (00188616)
• Co-Investigator(Kenkyū-buntansha): 高島 英造 愛媛大学, プロテオサイエンスセンター, 准教授 (50366762)
• Research Collaborator: SATTABONGKOT Jetsumon マヒドン大学, 熱帯医学部, 教授
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 原虫 / マラリア / サーベイランス / プロテインアレイ / タイ

Outline of Final Research Achievements

We have recently identified and validated a panel of serological markers able to detect recent exposure to P. vivax infection, thereby indirectly identifying malaria hot-spots. We screened 307 P. vivax proteins for their ability to induce IgG responses in P. vivax-exposed individuals in Thailand, Brazil, and Papua New Guines determined the kinetic profile of these responses over 9-months in the absence of any recurrent infections. Candidate markers were down-selected based on high immunogenicity at the time of infection, comparable antibody longevity between the three sites and low levels of individual variation in IgG responses. Finally, 55 down-selected proteins are the sero-marker candidates for the malaria hot-spot surveillance.

Research Products

• [Int'l Joint Research] Mahidol University(タイ)
• [Int'l Joint Research] Walter & Eliza Hall 医学研究所(オーストラリア)
• [Int'l Joint Research] PNG Institute of Medical Research(パプアニューギニア)
• [Int'l Joint Research] Burnet institute/Walter & Elisa Hall Institute(オーストラリア)
• [Int'l Joint Research] Mahidol University(タイ)
• [Int'l Joint Research] Mahidol University(Thailand)
• [Int'l Joint Research] Kangwon National University(韓国)
• [Journal Article] Naturally acquired antibody responses to more than 300 Plasmodium vivax proteins in three geographic regions (2017)
  • Author(s): Longley Rhea J.、White Michael T.、Takashima Eizo、Morita Masayuki、Kanoi Bernard N.、Li Wai Suen Connie S. N.、Betuela Inoni、Kuehn Andrea、Sripoorote Piyarat、Franca Camila T.、Siba Peter、Robinson Leanne J.、Lacerda Marcus、Sattabongkot Jetsumon、Tsuboi Takafumi、Mueller Ivo
  • Journal Title: PLOS Neglected Tropical Diseases Volume: 11 Issue: 9 Pages: e0005888-e0005888
  • DOI: 10.1371/journal.pntd.0005888
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of highly-protective combinations of Plasmodium vivax recombinant proteins for vaccine development (2017)
  • Author(s): Franca Camila, White Michael, Yadava Anjali, Siba Peter, Galinski Mary, Healer Julie, Chitnis Chetan, Cowman Alan, Takashima Eizo, Tsuboi Takafumi, Tham Wai-Hong, Fairhurst Rick, Rayner Julian, King Christopher, Mueller Ivo, 他9名
  • Journal Title: eLife Volume: 6
  • DOI: 10.7554/elife.28673
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Serological markers to measure recent changes in malaria at population level in Cambodia. (2016)
  • Author(s): Kerkhof K, Sluydts V, Willen L, Kim S, Canier L, Heng S, Tsuboi T, Sochantha T, Sovannaroth S, Menard D, Coosemans M, Durnez L.
  • Journal Title: Malar J. Volume: 15 Issue: 1 Pages: 529-529
  • DOI: 10.1186/s12936-016-1576-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of Plasmodium falciparum reticulocyte binding protein homologue 5-interacting protein, PfRipr, as a highly conserved blood-stage malaria vaccine candidate. (2016)
  • Author(s): Ntege EH, Arisue N, Ito D, Hasegawa T, Palacpac NM, Egwang TG, Horii T, Takashima E, Tsuboi T.
  • Journal Title: Vaccine Volume: 34 Issue: 46 Pages: 5612-5622
  • DOI: 10.1016/j.vaccine.2016.09.028
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Plasmodium vivax GPI-anchored micronemal antigen (PvGAMA) binds human erythrocytes independent of Duffy antigen status. (2016)
  • Author(s): Cheng Y, Lu F, Wang B, Li J, Han JH, Ito D, Kong DH, Jiang L, Wu J, Ha KS, Takashima E, Sattabongkot J, Cao J, Nyunt MH, Kyaw MP, Desai SA, Miller LH, Tsuboi T, Han ET.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 35581-35581
  • DOI: 10.1038/srep35581
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The association between naturally acquired IgG subclass specific antibodies to the PfRH5 invasion complex and protection from Plasmodium falciparum malaria. (2016)
  • Author(s): Weaver R, Reiling L, Feng G, Drew DR, Mueller I, Siba PM, Tsuboi T, Richards JS, Fowkes FJ, Beeson JG.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 33094-33094
  • DOI: 10.1038/srep33094
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Antibody responses to Plasmodium falciparum and Plasmodium vivax blood-stage and sporozoite antigens in the postpartum period. (2016)
  • Author(s): McLean AR, Boel ME, McGready R, Ataide R, Drew D, Tsuboi T, Beeson JG, Nosten F, Simpson JA, Fowkes FJ.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 32159-32159
  • DOI: 10.1038/srep32159
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Plasmodium vivax gametocyte proteins, Pvs48/45 and Pvs47, induce transmission-reducing antibodies by DNA immunization (2015)
  • Author(s): Tachibana M, Suwanabun N, Kaneko O, Iriko H, Otsuki H, Sattabongkot J, Kaneko A, Herrera S, Torii M, Tsuboi T
  • Journal Title: Vaccine Volume: 33 Issue: 16 Pages: 1901-1908
  • DOI: 10.1016/j.vaccine.2015.03.008
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Rodent malaria lactate dehydrogenase assay provides a high throughput solution for in vivo vaccine studies (2015)
  • Author(s): Otsuki H, Yokouchi Y, Iyoku N, Tachibana M, Tsuboi T, Torii M
  • Journal Title: Parasitol Int Volume: 64 Issue: 4 Pages: 60-63
  • DOI: 10.1016/j.parint.2015.02.001
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Immunoprofiling of the tryptophan-rich antigen family in Plasmodium vivax (2015)
  • Author(s): Wang B, Lu F, Cheng Y, Chen JH, Jeon HY, Ha KS, Cao J, Nyunt MH, Han JH, Lee SK, Kyaw MP, Sattabongkot J, Takashima E, Tsuboi T, Han ET
  • Journal Title: Infect Immun Volume: 83 Issue: 8 Pages: 3083-3095
  • DOI: 10.1128/iai.03067-14
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 2030 年のマラリア排除に向けた日本の貢献-アカデミアは何ができるか？ (2018)
  • Author(s): 坪井敬文
  • Organizer: 第87回日本寄生虫学会大会
  • Invited
• [Presentation] Post-genome malaria vaccine research. (2017)
  • Author(s): Tsuboi T
  • Organizer: Japan-Brazil Malaria Research Workshop: fostering new partnerships
  • Place of Presentation: Sao Paulo, Brazil
  • Year and Date: 2017-03-15
  • Int'l Joint Research / Invited
• [Presentation] A vaccine to block malaria transmission: Pfs230 antigen design and display (2017)
  • Author(s): Tsuboi T
  • Organizer: GHIT R&D Forum
  • Int'l Joint Research / Invited
• [Presentation] ポストゲノムのマラリアワクチン研究 (2017)
  • Author(s): 坪井敬文
  • Organizer: 第30回日本動物細胞工学会2017年度大会
  • Invited
• [Presentation] ナニワからマラリア克服へ (2017)
  • Author(s): 坪井敬文
  • Organizer: 第73回日本寄生虫学会西日本支部大会
  • Invited
• [Presentation] Post-genome malaria vaccine candidate discovery by wheat germ cell-free system. (2016)
  • Author(s): Tsuboi T
  • Organizer: Joint International Tropical Medicine Meeting 2016
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2016-12-07
  • Int'l Joint Research / Invited
• [Presentation] Reverse vaccinology approach: Postgenome malaria blood-stage vaccine candidate discovery. (2016)
  • Author(s): Tsuboi T, Ntege EH, Takashima E, Morita M, Ito D, Cowman A
  • Organizer: International Congress for Tropical Medicine and Malaria 2016
  • Place of Presentation: Brisbane, Australia
  • Year and Date: 2016-09-18
  • Int'l Joint Research
• [Presentation] Approaches to malaria vaccine development. (2016)
  • Author(s): Tsuboi T
  • Organizer: Workshop on Tools for Malaria Eradication
  • Place of Presentation: RIKEN Yokohama Campus, Yokohama, Kanagawa, Japan
  • Year and Date: 2016-06-06
  • Int'l Joint Research / Invited
• [Presentation] ポストゲノムマラリアワクチン研究は宿主寄生体関係の総体的理解につながる (2015)
  • Author(s): 坪井敬文
  • Organizer: 第88回日本生化学会大会・第38回日本分子生物学会年会 合同大会
  • Place of Presentation: 神戸ポートアイランド、神戸市、兵庫県
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] Pre-erythrocytic vaccines for malaria elimination (2015)
  • Author(s): Tsuboi T
  • Organizer: Malaria R&D in a Time of Global Partnerships
  • Place of Presentation: 東京大学、東京都
  • Year and Date: 2015-06-26
  • Int'l Joint Research / Invited
• [Presentation] An integrated approach to tackling malaria in Uganda with special reference to novel malaria vaccine candidate discovery (2015)
  • Author(s): Tsuboi T, Kanoi BN, Egwang TG, Horii T
  • Organizer: E-JUST 2nd International Conference on Innovative Engineering
  • Place of Presentation: Bibliotheca Alexandria, Alexandria, Egypt
  • Year and Date: 2015-05-19
  • Int'l Joint Research / Invited
• [Presentation] WGCFS: an innovative technology for post-genome malaria vaccine research (2015)
  • Author(s): Tsuboi T
  • Organizer: 25th Annual Molecular Parasitology/Vector Biology Symposium
  • Place of Presentation: University of Georgia, Athens, USA
  • Year and Date: 2015-04-28
  • Int'l Joint Research / Invited
• [Remarks] 愛媛大学プロテオサイエンスセンター マラリア研究部門
  • URL: http://www.pros.ehime-u.ac.jp/malaria/
• [Remarks] 愛媛大学プロテオサイエンスセンターマラリア研究部門
  • URL: http://www.pros.ehime-u.ac.jp/malaria/
• [Remarks] 愛媛大学プロテオサイエンスセンター マラリア研究部門
  • URL: http://www.pros.ehime-u.ac.jp/malaria/