Characterizing avian influenza H5N1 viruses in Egypt
| Project/Area Number |
15H05287
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Hygiene and public health
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
NAKAYA Takaaki 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80271633)
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| Co-Investigator(Kenkyū-buntansha) |
開發 邦宏 大阪大学, 産業科学研究所, 特任准教授(常勤) (70419464)
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| Co-Investigator(Renkei-kenkyūsha) |
DAIDOJI Tomo 京都府立医科大学, 大学院医学研究科, 講師 (80432433)
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| Research Collaborator |
Ibrahim Madiha S. エジプト・ダマンフール大学, 獣医学部, 教授
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | インフルエンザ / H5N1 / エジプト / ペプチド核酸 / ウイルス / 検出系 / Ｈ５Ｎ１ |
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| Outline of Final Research Achievements |
We reported that highly pathogenic avian influenza H5N1 viruses, isolated from naturally infected pigeons in Egypt, have several unique mutations in viral polymerase genes. We also demonstrated that these mutations were responsible for changes in polymerase activity, providing new insights into the possible roles of pigeons in avian influenza virus transmission and evolution. We further determined hemagglutinin genetic diversification that occurred during H5N1 virus infection of primary human airway cells and characterized their phenotypes by recombinant variants. Our results revealed that a variety of minor HA variants emerged during a single infection in human airway cells, which altered their HA properties to more efficiently infect human cells.
In addition, we prepared a novel hairpin-type peptide nucleic acid, which has a sequence complementary to the N1-NA gene of influenza A virus, and demonstrated the sequence-dependent detection of N1 subtype viruses.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Characterization of H5N1 influenza virus quasispecies with adaptive hemagglutinin mutations from single-virus infections of human airway cells2018
Author(s)
1.Watanabe Y**, Arai Y, Kawashita N, Ibrahim MS, Elgendy EM, Daidoji T, Kajikawa J, Hiramatsu H, Sriwilaijaroen N, Ono T, Takagi T, Takahashi K, Shioda T, Matsumoto K, Suzuki Y, Nakaya T
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Journal Title
J Virol.
Volume: 印刷中
Issue: 11
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Influenza A Virus-Induced Expression of a GalNAc Transferase, GALNT3, via MicroRNAs Is Required for Enhanced Viral Replication.2015
Author(s)
Nakamura S, Horie M, Daidoji T, Honda T, Yasugi M, Kuno A, Komori T, Okuzaki D, Narimatsu H, Nakaya T, Tomonaga K.
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Journal Title
J Virol.
Volume: 90
Issue: 4
Pages: 1788-801
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Characterization of H5N1 Influenza Virus Variants with Hemagglutinin Mutations Isolated from Patients.2015
Author(s)
1.Watanabe Y, Arai Y, Daidoji T, Kawashita N, Ibrahim MS, El-Gendy E, Hiramatsu H, Kubota-Kokestu R, Takagi T, Murata T, Takahashi K, Okuno Y, Nakaya T, Suzuki Y, Ikuta K.
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Avian Influenza Virus Infection of Immortalized Human Respiratory Epithelial Cells Depends upon a Delicate Balance between Hemagglutinin Acid Stability and Endosomal pH.2015
Author(s)
3.Daidoji T, Watanabe Y, Ibrahim MS, Yasugi M, Maruyama H, Masuda T, Arai F, Ohba T, Honda A, Ikuta K, Nakaya T
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Journal Title
J Biol Chem
Volume: 290
Issue: 17
Pages: 10627-42
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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