Dissection and reconstitution of ultradian genetic oscillators responsive to dynamic signaling inputs
| Project/Area Number |
15H05326
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Life / Health / Medical informatics
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| Research Institution | Kyoto University |
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Principal Investigator |
Isomura Akihiro 京都大学, ウイルス・再生医科学研究所, 共同研究者 (70512466)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥24,700,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥5,700,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
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| Keywords | 遺伝子発現振動 / 光遺伝学 / 人工遺伝子回路 / 合成生物学 / 非線形動力学 / システム生物学 / 生物物理 |
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| Outline of Final Research Achievements |
We studied dynamic responses of Hes1 genetic oscillators via optogenetic perturbation and single-cell imaging analysis. We found that Hes1 oscillators can decode dynamic signaling inputs at the single-cell level. We also succeeded in the visualization of single-cell phase response curves. Numerical simulations suggested that stochastic phase model is sufficient to explain synchronization processes of Hes1 oscillators upon external periodic perturbation.
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Report
(4 results)
Research Products
(7 results)
