| Budget Amount *help |
¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Outline of Final Research Achievements |
In this study, to clarify the correlation between the fold-specific substrate recognition and the function of the private chaperones, the system for generation of the synthetic binding proteins that specifically bind to the target proteins has been developed. The phage display libraries of the synthetic binding proteins using single-chain monellin as a non-antibody molecular scaffold were designed and constructed. By sorting the phage display libraries against the green fluorescent protein variant (GFPuv), the synthetic binding protein that has the ability to bind to GFPuv (GBP-1) was generated. The characteristics of GBP-1 revealed in this study indicate that single-chain monellin is useful as a non-antibody molecule backbone of the synthetic binding proteins.
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