| Budget Amount *help |
¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
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| Outline of Final Research Achievements |
The aim of this project is to reveal how the receptive fields of higher-order visual cortical areas (HVAs) are shaped by the thalamus. We identified synaptic input to the V1 and each HVA by injecting retrograde viruses into each visual area of mice. Neurons of the lateral geniculate nucleus (LGN) neurons project to both the V1 and various HVAs, whereas neurons of the lateral posterior nucleus (LP) project to various HVAs, but not to the V1. We imaged visual responses from axon terminals of the LP or LGN neurons expressing GCaMP6 by 2-photon microscopy. In addition, we imaged visual responses from the V1 or HVA while manipulating activity of neurons in the LP or LGN with the Designer Receptors Exclusively Activated by Designer Drug (DREADD) system. From these results, we conclude that LP neurons play a pivotal role in formation of the receptive fields of HVA neurons in mice.
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