Development of time-resolved spectroscopic systems with micro-flow devices and their application to the molecular mechanism of ABC transporters
| Project/Area Number |
15H05476
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical chemistry
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| Research Institution | Kobe University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TSUBAKI Motonari 神戸大学, 大学院理学研究科, 教授 (00145046)
SUGIMOTO Hiroshi 理化学研究所, 放射光科学研究センター, 専任研究員 (90344043)
SHIRO Yoshitsugu 兵庫県立大学, 大学院生命理学研究科, 教授 (70183051)
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| Research Collaborator |
HAYASHI Sae
ONISHI Kizashi
SHIMONAKA Misato
FUJII Koichi
TAKEDA Hanae
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
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| Keywords | 時間分解分光法 / マイクロ流路デバイス / 顕微分光 / X線自由電子レーザー / 赤外分光法 / 時間分解計測 / ABCトランスポーター / 顕微分光測定 / ダイナミクス / ヘム / マイクロ流体デバイス / 時間分解測定 / 分光法 / 膜タンパク質 / タンパク質ダイナミクス / 赤外吸収分光法 / 可視吸収分光法 / 蛍光寿命測定 / マイクロデバイス |
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| Outline of Final Research Achievements |
The novel time-resolved measurement systems that are applicable to many proteins have been developed by combining micro-fluidic techniques and spectroscopic measurements. In addition, time-resolved serial-femtosecond x-ray crystallography was developed by using the micro-fluidic mixer, allowing us to monitor the conformational changes in protein during the expression of functions in real-time manner. These novel measurement systems were applied to the ABC transporter, clarifying its transport and structural dynamics.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] A Nearly On-axis Spectroscopic System for Simultaneously Measuring UV-visible Absorption and X-ray Diffraction in the SPring-8 Structural Genomics Beamline2016
Author(s)
M. Sakaguchi, T. Kimura, T. Nishida, T. Tosha, Y. Yamaguchi, S. Yanagisawa, G. Ueno, H. Murakami, H. Ago, M. Yamamoto, T. Ogura, Y. Shiro, M. Kubo
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Journal Title
J. Synchro. Rad.
Volume: 23
Issue: 1
Pages: 334-338
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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