Establishment of bioluminescence sensor and imaging system for monitoring cell to cell communication
| Project/Area Number |
15H05488
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | University of Fukui (2016)
The University of Tokyo (2015) |
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Principal Investigator |
Hattori Mitsuru 福井大学, 学術研究院医学系部門, 助教 (20589858)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2016: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2015: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
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| Keywords | ルシフェラーゼ / 生物発光 / イメージング / タンパク質 / バイオセンサー / コミュニケーション |
|---|
| Outline of Final Research Achievements |
We developed a new bioluminescence tool for monitoring protein dynamics in living cells including cell to cell communication, with newly constructed a microscope observation system. For tracing the temporal changing of cell activity rapidly, we choose the novel luciferase species that has sufficiently high luminescence intensity. We have already established an optical technique for monitoring protein-protein interactions in living cells based on complementation of split fragments of luciferase. In this case, the luciferase was also used as split fragments to monitor the protein that plays a key role in target phenomenon. We also developed a bioluminescence observation system based inverted fluorescence microscope, the set-up in which was with high sensitive and darkness condition. Finally, the luminescent probe in combination with the observation system monitored GPCR activities and the temporal correlation between cell and cell.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Spatiotemporal analysis with a genetically encoded fluorescent RNA probe reveals TERRA function around telomeres.2016
Author(s)
Yamada, T., Yoshimura, H., Shimada, R., Hattori, M., Eguchi, T., Fujiwara, K., Kusumi, A. and Ozawa, T.
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Journal Title
Sci. Rep.
Volume: 6
Issue: 1
Pages: 38910-38910
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Confocal Bioluminescence Imaging for Living Tissues with a Caged Substrate of Luciferin.2016
Author(s)
Hattori, M., Kawamura, G., Kojima, R., Kamiya, M., Urano, Y. and Ozawa, T.
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Journal Title
Anal. Chem.
Volume: 88(12)
Issue: 12
Pages: 6231-6238
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Optogenetic activation of axon guidance receptors controls direction of neurite outgrowth2016
Author(s)
Endo, M., Hattori,M., Toriyabe, H., Ohno, H., Kamiguchi, H., Iino, Y. and Ozawa, T.
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Journal Title
Sci. Rep.
Volume: 6
Issue: 1
Pages: 23976-23976
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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