Interaction of membrane proteins on asymmetric lipid membrane toward creation of artificial translocon
| Project/Area Number |
15H05493
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bio-related chemistry
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| Research Institution | Kanagawa Institute of Industrial Sclence and Technology (2017)
Kanagawa Academy of Science and Technology (2015-2016) |
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Principal Investigator |
Kamiya Koki 地方独立行政法人神奈川県立産業技術総合研究所, 人工細胞膜システムグループ, 研究員(任期有) (70612315)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
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| Keywords | リポソーム / リン脂質非対称膜 / 膜タンパク質 / マイクロ流体デバイス / 人工細胞モデル / 人工細胞 / マイクロデバイス / ベシクル / 無細胞タンパク質合成系 |
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| Outline of Final Research Achievements |
In this study, I investigated interactions between asymmetric lipid membranes and biological molecules (peptides or membrane proteins). The asymmetric lipid vesicles were generated from a planar lipid bilayer by applying the pulsed jet flow. I achieved three items. Frist, I found that the reconstruction amounts of the membrane proteins into the lipid membranes changed by the lipid compositions, Second, I developed a device for sequentially generating various types of asymmetric lipid vesicles. Third, I developed a well-controlled cell or liposome-trapping device.
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Report
(4 results)
Research Products
(29 results)
