| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2017: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2016: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2015: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Outline of Final Research Achievements |
To identify novel biotribological molecules for articular cartilage, a newly system which is able to detect both bio-chemical and physical interactions between them was developed using surface plasmon resonance (SPR). In this system, molecules involving in articular lubrication which can interact with lubricin or hyaluronic acid were initially identified using molecular biological methods, and then selected according to whether those molecules can affect mechanical interactions at the material interface. To detect unknown time-dependent interaction between molecules in real-time using SPR, the incident light had a certain distribution of incident angle, which could avoid a delay attributed to the mechanical scanning of the SPR angle. In addition, to concurrently detect the mechanical interaction between molecules on this SPR device, grafted molecule on the gold film using self-assembled monolayer was applied.
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