Study on the signal transduction modulated by membrane potential
Project/Area Number |
15H05593
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Biophysics
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Research Institution | Kyushu Institute of Technology (2017) Institute of Physical and Chemical Research (2015-2016) |
Principal Investigator |
Morimoto Yusuke 九州工業大学, 大学院情報工学研究院, 助教 (50631777)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2015: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
|
Keywords | シグナル伝達 / 膜電位 / 走化性 / 蛍光イメージング / 光操作 / 生物物理 / 細胞運動 |
Outline of Final Research Achievements |
Plasma membrane potential is required for various cellular functions. The social amoebae Dictyostelium discoideum has been studied as a eukaryotic model organism to investigate chemotaxis, directed cell migration and differentiation. Chemotactic stimulation by cAMP is known to elicit cationic fluxes, involving proton and calcium ion, across the plasma membrane in Dictyostelium cells. This suggests that the membrane potential is also changed by cAMP stimulation. However it remains unknown how the membrane potential works in the eukaryotic signal transduction. In this study, we established the method to monitor the plasma membrane potential of Dictyostelium cells by fluorescence microscopy using a highly sensitive membrane potential dye. Then we found that the membrane potential of Dictyostelium cells was periodically changed depending on the cAMP signal relay in the aggregation stage. Results indicate that the membrane potential change regulate the cAMP signaling relay.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] Tunnel Formation Inferred from the I-Form Structures of the Proton-Driven Protein Secretion Motor SecDF2017
Author(s)
Arata Furukawa, Kunihito Yoshikaie, Takaharu Mori, Hiroyuki Mori, Yusuke V. Morimoto, Yasunori Sugano, Shigehiro Iwaki, Tohru Minamino, Yuji Sugita, Yoshiki Tanaka, Tomoya Tsukazaki
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Journal Title
Cell Reports
Volume: 19
Issue: 5
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Domain-swap polymerization drives the self-assembly of the bacterial flagellar motor2016
Author(s)
Matthew A.B. Baker, Robert M.G. Hynson, Lorraine A. Ganuelas, Nasim Shah Mohammadi, Chu Wai Liew, Anthony A. Rey, Anthony P. Duff, Andrew E. Whitten, Cy M. Jeffries, Nicolas J. Delalez, Yusuke V. Morimoto, Daniela Stock, Judith P. Armitage, Andrew J. Turberfield, Keiichi Namba, Richard M. Berry and Lawrence K. Lee
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Journal Title
Nature Structural & Molecular Biology
Volume: 23
Issue: 3
Pages: 197-203
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] FliH and FliI ensure efficient energy coupling of flagellar type III protein export in Salmonella.2016
Author(s)
Minamino T, Kinoshita M, Inoue Y, Morimoto YV, Ihara K, Koya S, Hara N, Nishioka N, Kojima S, Homma M, Namba K
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Journal Title
Microbiologyopen
Volume: -
Issue: 3
Pages: 340-340
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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