The elucidation of the relationship between the formation of stromule and salinity tolerance by 3D reconstruction
| Project/Area Number |
15H05613
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Crop production science
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| Research Institution | Kindai University |
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Principal Investigator |
YAMANE Koji 近畿大学, 農学部, 准教授 (50580859)
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| Research Collaborator |
TANIGUCHI Mitsutaka 名古屋大学, 生命農学研究科, 教授 (40231419)
OI Takao 名古屋大学, 生命農学研究科, 助教 (60752219)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2015: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
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| Keywords | イネ / 塩ストレス / 葉緑体 / 三次元再構築法 / 立体像 / 3次元構造 / 電子顕微鏡 / FIB-SEM |
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| Outline of Final Research Achievements |
The invagination structure of a chloroplast that surrounds organelles such as mitochondria and peroxisomes within a thin layer of chloroplast stroma is called a chloroplast pocket. Since it is expected that stromules, which are long tubules including stroma, might be involved in the formation, we constructed the three-dimensional architecture of chloroplast pockets by using serial images obtained by TEM and FIB-SEM. Three types of chloroplast pockets were observed by TEM: organelles were completely enclosed in a chloroplast pocket (enclosed type), a chloroplast pocket with a small gap in the middle part (gap type), and a chloroplast pocket with one side open (open type). Mitochondria and peroxisomes were often in contact with the chloroplast pockets. FIB-SEM revealed chloroplasts with a sheet structure partially surrounding peroxisomes. This fact suggests that chloroplasts might construct large sheet structures that would be related to the formation of chloroplast pockets.
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Report
(4 results)
Research Products
(9 results)
