Archiving of the symbionts of arthropods for controlling vector-borne diseases

• Project/Area Number: 15H05633
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: Nakao Ryo 北海道大学, 獣医学研究院, 准教授 (50633955)
• Research Collaborator: ABE takashi ; TANAKA tetsuya
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000); Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2015: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 節足動物 / 共生微生物 / 細菌叢 / マダニ / ベクター / 原虫 / コクシエラ / リケッチア / 真核生物叢 / Rickettsia / Spiroplasma / ペプチド核酸 / ゲノム / メタゲノム / 共生 / 微生物叢 / Coxiella / Coxiella様共生菌 / 病原体 / クラミジア

Outline of Final Research Achievements

Some of the arthropods such as ticks can carry pathogens to humans and animals. The present study was aimed to disclose the diverse microbiome in such vector arthropods. We found that some bacteria such as Coxiella and Rickettsia dominantly exist in ticks. Comparison between tick phylogeny and bacterial genotypes revealed that ticks have acquired the dominant bacteria via both vertical and horizontal transmission.

Academic Significance and Societal Importance of the Research Achievements

マダニが保有する微生物群全体を解析することで、新規のものを含む多数の微生物の検出に成功した。それら微生物の遺伝子情報は、マダニがどのようにして微生物の助けを得ながら生存しているのかを知る鍵となる。微生物がマダニ集団内でどのように維持されているかを知ることは、マダニがどのように病原体を獲得しているのかを知ることにつながり、マダニが媒介する感染症の対策に応用できる。

Research Products

• [Int'l Joint Research] University of Zambia(ザンビア)
• [Int'l Joint Research] University of Veterinary Science(ミャンマー)
• [Int'l Joint Research] Freie Universitat Berlin(ドイツ)
• [Int'l Joint Research] University of Liverpool(英国)
• [Int'l Joint Research] University of Khartoum(スーダン)
• [Int'l Joint Research] University of Zambia(Zambia)
• [Int'l Joint Research] University of Veterinary Science(Myanmar)
• [Int'l Joint Research] Freie Universitat Berlin(Germany)
• [Int'l Joint Research] University of Liverpool(英国)
• [Int'l Joint Research] University of Zambia(Zambia)
• [Int'l Joint Research] University of Veterinary Science(Myanmar)
• [Int'l Joint Research] Freie Universitat Berlin(Germany)
• [Int'l Joint Research] University of Zambia(Zambia)
• [Int'l Joint Research] University of Veterinary Science(Myanmar)
• [Journal Article] Molecular detection of Rickettsia felis in dogs, rodents and cat fleas in Zambia (2019)
  • Author(s): Moonga Lavel Chinyama、Hayashida Kyoko、Nakao Ryo、Lisulo Malimba、Kaneko Chiho、Nakamura Ichiro、Eshita Yuki、Mweene Aaron S.、Namangala Boniface、Sugimoto Chihiro、Yamagishi Junya
  • Journal Title: Parasites & Vectors Volume: 12 Issue: 1 Pages: 168-168
  • DOI: 10.1186/s13071-019-3435-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Potential role of dogs as sentinels and reservoirs for piroplasms infecting equine and cattle in Riyadh City, Saudi Arabia (2019)
  • Author(s): Salim Bashir、Alanazi Abdullah D.、Omori Ryosuke、Alyousif Mohamed S.、Alanazi Ibrahim O.、Katakura Ken、Nakao Ryo
  • Journal Title: Acta Tropica Volume: 193 Pages: 78-83
  • DOI: 10.1016/j.actatropica.2019.02.029
  • NAID: 120006843068
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular and phenotypic characterization of Leptospira johnsonii sp. nov., Leptospira ellinghausenii sp. nov. and Leptospira ryugenii sp. nov. isolated from soil and water in Japan (2019)
  • Author(s): Masuzawa Toshiyuki、Saito Mitsumasa、Nakao Ryo、Nikaido Yasuhiko、Matsumoto Masahiro、Ogawa Midori、Yokoyama Mitsuru、Hidaka Yusuke、Tomita Junko、Sakakibara Keiko、Suzuki Kazushi、Yasuda Shima、Sato Hiroki、Yamaguchi Masaya、Yoshida Shin‐ichi、Koizumi Nobuo、Kawamura Yoshiaki
  • Journal Title: Microbiology and Immunology Volume: 63 Issue: 3-4 Pages: 89-99
  • DOI: 10.1111/1348-0421.12671
  • Peer Reviewed
• [Journal Article] Isolation of Rickettsia, Rickettsiella, and Spiroplasma from Questing Ticks in Japan Using Arthropod Cells (2019)
  • Author(s): Thu May June、Qiu Yongjin、Kataoka-Nakamura Chikako、Sugimoto Chihiro、Katakura Ken、Isoda Norikazu、Nakao Ryo
  • Journal Title: Vector-Borne and Zoonotic Diseases Volume: 印刷中 Issue: 7 Pages: 474-485
  • DOI: 10.1089/vbz.2018.2373
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Diversity of spotted fever group rickettsiae and their association with host ticks in Japan (2019)
  • Author(s): Thu MJ, Qiu Y, Matsuno K, Kajihara M, Mori-Kajihara A, Omori R, Monma N, Chiba K, Seto J, Gokuden M, Andoh M, Oosako H, Katakura K, Takada A, Sugimoto C, Isoda N, Nakao R
  • Journal Title: Scientific Reports Volume: 9(1) Issue: 1 Pages: 1500-1500
  • DOI: 10.1038/s41598-018-37836-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Viral population analysis of the taiga tick, <i>Ixodes persulcatus,</i> by using Batch Learning Self-Organizing Maps and BLAST search (2019)
  • Author(s): QIU Yongjin、ABE Takashi、NAKAO Ryo、SATOH Kenro、SUGIMOTO Chihiro
  • Journal Title: Journal of Veterinary Medical Science Volume: 81 Issue: 3 Pages: 401-410
  • DOI: 10.1292/jvms.18-0483
  • NAID: 130007617731
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evidence of multiple point mutations in Theileria annulata cytochrome b gene incriminated in buparvaquone treatment failure (2019)
  • Author(s): Chatanga Elisha、Mosssad Ehab、Abdo Abubaker Hazem、Amin Alnour Sondos、Katakura Ken、Nakao Ryo、Salim Bashir
  • Journal Title: Acta Tropica Volume: 191 Pages: 128-132
  • DOI: 10.1016/j.actatropica.2018.12.041
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Infection of newly identified phleboviruses in ticks and wild animals in Hokkaido, Japan indicating tick-borne life cycles (2019)
  • Author(s): Torii Shiho、Matsuno Keita、Qiu Yongjin、Mori-Kajihara Akina、Kajihara Masahiro、Nakao Ryo、Nao Naganori、Okazaki Katsunori、Sashika Mariko、Hiono Takahiro、Okamatsu Masatoshi、Sakoda Yoshihiro、Ebihara Hideki、Takada Ayato、Sawa Hirofumi
  • Journal Title: Ticks and Tick-borne Diseases Volume: 10 Issue: 2 Pages: 328-335
  • DOI: 10.1016/j.ttbdis.2018.11.012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Recombination and purifying and balancing selection determine the evolution of major antigenic protein 1 (map 1) family genes in Ehrlichia ruminantium. (2019)
  • Author(s): Salim B, Amin M, Igarashi M, Ito K, Jongejan F, Katakura K, Sugimoto C, Nakao R.
  • Journal Title: Gene Volume: 683 Pages: 216-224
  • DOI: 10.1016/j.gene.2018.10.028
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Human Borreliosis Caused by a New World Relapsing Fever Borrelia-like Organism in the Old World (2018)
  • Author(s): Qiu Yongjin、Nakao Ryo、Hang’ombe Bernard Mudenda、Sato Kozue、Kajihara Masahiro、Kanchela Sharon、Changula Katendi、Eto Yoshiki、Ndebe Joseph、Sasaki Michihito、Thu May June、Takada Ayato、Sawa Hirohumi、Sugimoto Chihiro、Kawabata Hiroki
  • Journal Title: Clinical Infectious Diseases Volume: 印刷中 Issue: 1 Pages: 107-112
  • DOI: 10.1093/cid/ciy850
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Development and validation of direct dry loop mediated isothermal amplification for diagnosis of Trypanosoma evansi (2018)
  • Author(s): Salim Bashir、Hayashida Kyoko、Mossaad Ehab、Nakao Ryo、Yamagishi Junya、Sugimoto Chihiro
  • Journal Title: Veterinary Parasitology Volume: 260 Pages: 53-57
  • DOI: 10.1016/j.vetpar.2018.08.009
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] First molecular detection of Theileria luwenshuni from goats in Myanmar (2018)
  • Author(s): Bawm Saw、Kakisaka Keita、Thu May June、Chel Hla Myet、Oo Yamon Min Nyunt、Soe Nyein Chan、Win Shwe Yee、Htun Lat Lat、Win Mar Mar、Suzuki Hitoshi、Nakao Ryo、Katakura Ken
  • Journal Title: Parasitology Research Volume: 117 Issue: 10 Pages: 3361-3364
  • DOI: 10.1007/s00436-018-6073-6
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Analysis for genetic loci controlling protoscolex development in the Echinococcus multilocularis infection using congenic mice. (2018)
  • Author(s): Islam Md Atiqul, Torigoe Daisuke, Kameda Yayoi, Irie Takao, Kouguchi Hirokazu, Nakao Ryo, Masum Md Abdul, Ichii Osamu, Kon Yasuhiro, Tag-El-Din-Hassan, Morimatsu Masami, Yagi Kinpei, Agui Takashi.
  • Journal Title: Infection, Genetics and Evolution Volume: 65 Pages: 65-71
  • DOI: 10.1016/j.meegid.2018.07.017
  • NAID: 120006764182
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Comparison of Database Search Methods for the Detection of Legionella pneumophila in Water Samples Using Metagenomic Analysis (2018)
  • Author(s): Borthong Jednipit、Omori Ryosuke、Sugimoto Chihiro、Suthienkul Orasa、Nakao Ryo、Ito Kimihito
  • Journal Title: Frontiers in Microbiology Volume: 9
  • DOI: 10.3389/fmicb.2018.01272
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Unique Phylogenetic Position of a Novel Tick-Borne Phlebovirus Ensures an Ixodid Origin of the Genus Phlebovirus (2018)
  • Author(s): Matsuno Keita、Kajihara Masahiro、Nakao Ryo、Nao Naganori、Mori-Kajihara Akina、Muramatsu Mieko、Qiu Yongjin、Torii Shiho、Igarashi Manabu、Kasajima Nodoka、Mizuma Keita、Yoshii Kentaro、Sawa Hirofumi、Sugimoto Chihiro、Takada Ayato、Ebihara Hideki
  • Journal Title: mSphere Volume: 3 Issue: 3
  • DOI: 10.1128/msphere.00239-18
  • NAID: 120006501204
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Seroprevalence of Filovirus Infection of Rousettus aegyptiacus Bats in Zambia (2018)
  • Author(s): Changula K, Kajihara M, Mori-Kajihara A, Eto Y, Miyamoto H, Yoshida R, Shigeno A, Hang'ombe B, Qiu Y, Mwizabi D, Squarre D, Ndebe J, Ogawa H, Harima H, Simulundu E, Moonga L, Kapila P, Furuyama W, Kondoh T, Sato M, Takadate Y, Kaneko C, Nakao R, Mukonka V, Mweene A, Takada A
  • Journal Title: J Infect Dis Volume: 218(Suppl 5)
  • DOI: 10.1093/infdis/jiy266
  • NAID: 120006771013
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Gene expression profiles of the small intestinal mucosa of dogs repeatedly infected with the cestode Echinococcus multilocularis (2018)
  • Author(s): Kouguchi, H., Irie, T., Matsumoto, J., Furuoka, H., Ishiwata, K., Nakao, R., and Yagi, K.
  • Journal Title: Data in Brief Volume: 17 Pages: 180-183
  • DOI: 10.1016/j.dib.2018.01.004
  • Peer Reviewed / Open Access
• [Journal Article] Potential of cell-free DNA as a screening marker for parasite infections in dog (2018)
  • Author(s): Akter Shirin、Nakao Ryo、Imasato Yuhei、Alam Mohammad Zahangir、Katakura Ken
  • Journal Title: Genomics Volume: - Issue: 4 Pages: 906-912
  • DOI: 10.1016/j.ygeno.2018.05.020
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tick-borne haemoparasites and Anaplasmataceae in domestic dogs in Zambia (2018)
  • Author(s): Qiu Yongjin、Kaneko Chiho、Kajihara Masahiro、Ngonda Saasa、Simulundu Edgar、Muleya Walter、Thu May J、Hang’ombe Bernard、Katakura Ken、Takada Ayato、Sawa Hirofumi、Simuunza Martin、Nakao Ryo
  • Journal Title: Ticks and Tick-borne Diseases Volume: 印刷中 Issue: 4 Pages: 988-995
  • DOI: 10.1016/j.ttbdis.2018.03.025
  • NAID: 120006626572
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] First molecular detection and genetic characterization of Coxiella burnetii in Zambian dogs and rodents (2018)
  • Author(s): Chitanga Simbarashe、Simulundu Edgar、Simuunza Martin、Changula Katendi、Qiu Yongjin、Kajihara Masahiro、Nakao Ryo、Syakalima Michelo、Takada Ayato、Mweene S Aaron、Mukaratirwa Samson、Hang’ombe Bernard
  • Journal Title: Parasites & Vectors Volume: 11 Issue: 1 Pages: 40-40
  • DOI: 10.1186/s13071-018-2629-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular detection and characterization of zoonotic Anaplasma species in domestic dogs in Lusaka, Zambia (2018)
  • Author(s): Vlahakis Pipina、Chitanga Simbarashe、Simuunza Martin、Simulundu Edgar、Qiu Yongjin、Changula Katendi、Chambaro Herman、Kajihara Masahiro、Nakao Ryo、Takada Ayato、Mweene Aaron
  • Journal Title: Ticks and Tick-borne Diseases Volume: 9 Issue: 1 Pages: 39-43
  • DOI: 10.1016/j.ttbdis.2017.10.010
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Geographic strain differentiation of Schistosoma japonicum in the Philippines using microsatellite markers. (2017)
  • Author(s): Moendeg KJ, Angeles JMM, Nakao R, Leonardo LR, Fontanilla IKC, Goto Y, Kirinoki M, Villacorte EA, Rivera PT, Inoue N, Chigusa Y, Kawazu SI
  • Journal Title: PLoS Negl. Trop. Dis. Volume: 11 Issue: 7 Pages: e0005749-e0005749
  • DOI: 10.1371/journal.pntd.0005749
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular identification and characterization of piroplasm species in Hokkaido sika deer (Cervus Nippon yesoensis), Japan. (2017)
  • Author(s): Elbaz, E., Moustafa, M. A. M., Lee, K., Mohamed, W. M. A., Nakao, R., Shimozuru, M., Sashika, M., Younis, E. E. A., El-khodery, S. A. & Tsubota, T.
  • Journal Title: Tick and Tick-borne Diseases Volume: 8 Issue: 5 Pages: 802-807
  • DOI: 10.1016/j.ttbdis.2017.06.007
  • NAID: 120006491493
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] First molecular detection and characterization of Hepatozoon and Sarcosystis spp. in field mice and voles from Japan. (2017)
  • Author(s): Moustafa, M. A. M., Shimozuru, M., Mohamed, W., Taylor, K. R., Nakao, R., Sashika, M. & Tsubota, T.
  • Journal Title: Parasitology Research Volume: 116 Issue: 8 Pages: 2321-2325
  • DOI: 10.1007/s00436-017-5505-z
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] <i>Listeria monocytogenes</i> serotype 4b strains replicate in monocytes/macrophages more than the other serotypes (2017)
  • Author(s): Hasebe R, Nakao R, Ohnuma A, Yamasaki T, Sawa H, Takai S, Horiuchi M.
  • Journal Title: Journal of Veterinary Medical Science Volume: 79 Issue: 6 Pages: 962-969
  • DOI: 10.1292/jvms.16-0575
  • NAID: 130005695727
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Molecular characterization of Fasciola flukes obtained from wild sika deer and domestic cattle in Hokkaido, Japan (2017)
  • Author(s): Ichikawa-Seki Madoka、Shiroma Tomoko、Kariya Tatsuya、Nakao Ryo、Ohari Yuma、Hayashi Kei、Fukumoto Shinya
  • Journal Title: Parasitology International Volume: 66 Issue: 5 Pages: 519-521
  • DOI: 10.1016/j.parint.2017.04.005
  • Peer Reviewed / Open Access
• [Journal Article] Meteorological factors affecting scrub typhus occurrence: a retrospective study of Yamagata Prefecture, Japan, 1984-2014 (2017)
  • Author(s): Seto J, Suzuki Y, Nakao R, Otani K, Yahagi K, Mizuta K
  • Journal Title: Epidemiol Infect Volume: 145 Issue: 3 Pages: 462-470
  • DOI: 10.1017/s0950268816002430
  • Peer Reviewed
• [Journal Article] Putative RNA viral sequences detected in an Ixodes scapularis-derived cell line. (2016)
  • Author(s): Nakao R, Matsuno K, Qiu Y, Maruyama J, Eguchi N, Nao N, Kajihara M, Yoshii K, Sawa H, Takada A, Sugimoto C.
  • Journal Title: Ticks Tick Borne Dis. Volume: 8 Issue: 1 Pages: 103-111
  • DOI: 10.1016/j.ttbdis.2016.10.005
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Horizontally transferred genetic elements in the tsetse fly genome: an alignment-free clustering approach using batch learning self-organising map (BLSOM). (2016)
  • Author(s): Nakao R, Abe T, Funayama S, Sugimoto C.
  • Journal Title: BioMed Res Int. Volume: 2016 Pages: 3164624-3164624
  • DOI: 10.1155/2016/3164624
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular and serological evidence of Leishmania infection in stray dogs from visceral leishmaniasis-endemic areas of Bangladesh. (2016)
  • Author(s): Akter S, Alam MZ, Nakao R, Yasin MG, Kato H, Katakura K.
  • Journal Title: Am J Trop Med Hyg. Volume: 95 Issue: 4 Pages: 795-799
  • DOI: 10.4269/ajtmh.16-0151
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Dynamics, co-infections and characteristics of zoonotic tick-borne pathogens in Hokkaido small mammals, Japan (2016)
  • Author(s): Moustafa MA, Taylor K, Nakao R, Shimozuru M, Sashika M, Rosa R, Thu MJ, Rizzoli A, Tsubota T
  • Journal Title: Ticks Tick Borne Dis Volume: ７ Issue: 5 Pages: 922-928
  • DOI: 10.1016/j.ttbdis.2016.04.014
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Molecular detection of spotted fever group rickettsiae in Amblyomma variegatum ticks from Benin (2016)
  • Author(s): Adjou Moumouni PF, Terkawi MA, Jirapattharasate C, Cao S, Liu M, Nakao R, Umemiya-Shirafuji R, Yokoyama N, Sugimoto C, Fujisaki K, Suzuki H, Xuan X
  • Journal Title: Ticks Tick Borne Dis Volume: 7 Issue: 5 Pages: 828-833
  • DOI: 10.1016/j.ttbdis.2016.03.016
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Draft genome sequences of three strains of Ehrlichia ruminantium, a tick-borne pathogen of ruminants, isolated from Zimbabwe, The Gambia, and Ghana (2016)
  • Author(s): Nakao R, Jongejan F, Sugimoto C
  • Journal Title: Genome Announc Volume: ４ Issue: 3
  • DOI: 10.1128/genomea.00453-16
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular survey of Babesia infections in cattle from different areas of Myanmar (2016)
  • Author(s): Bawm S, Htun LL, Maw NN, Ngwe T, Tosa Y, Kon T, Kaneko C, Nakao R, Sakurai T, Kato H, Katakura K
  • Journal Title: Ticks Tick Borne Dis. Volume: ７ Issue: 1 Pages: 204-207
  • DOI: 10.1016/j.ttbdis.2015.10.010
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular evidence of spotted fever group rickettsiae and Anaplasmataceae from ticks and stray dogs in Bangladesh (2016)
  • Author(s): Qiu Y, Nakao R, Thu MJ, Akter S, Alam MZ, Kato S, Katakura K, Sugimoto C
  • Journal Title: Parasitol Res Volume: 115 Issue: 3 Pages: 949-955
  • DOI: 10.1007/s00436-015-4819-y
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Studies of trypanosomiasis in the Luangwa valley, north-eastern Zambia (2015)
  • Author(s): Laohasinnarong D, Goto Y, Asada M, Nakao R, Hayashida K, Kajino K, Kawazu S, Sugimoto C, Inoue N, Namangala B
  • Journal Title: Parasit Vectors Volume: 8 Issue: 1 Pages: 497-497
  • DOI: 10.1186/s13071-015-1112-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular characterization and specific detection of Anaplasma species (AP-sd) in sika deer and its first detection in wild brown bears and rodents in Hokkaido, Japan (2015)
  • Author(s): Moustafa MA, Lee K, Taylor K, Nakao R, Sashika M, Shimozuru M, Tsubota T
  • Journal Title: Infect Genet Evol Volume: 36 Pages: 268-274
  • DOI: 10.1016/j.meegid.2015.09.027
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Morphological and molecular characterization of strongylid nematodes in Asian elephants of Myanmar (2019)
  • Author(s): Hla Myet Chel, May June Thu, Lat Lat Htun, Saw Bawm, Ryo Nakao, Ken Katakura
  • Organizer: 第88回日本寄生虫学会大会
• [Presentation] Genome editing in Echinococcus multilocularis using the CRISPR-Cas9 system (2019)
  • Author(s): Naoki Hayashi, Rumi Sagara, Takao Irie, Hirokazu Kouguchi, Kinpei Yagi, Ryo Nakao, Ken Katakura
  • Organizer: 第88回日本寄生虫学会大会
• [Presentation] Tick symbionts: Their diversity and evolutionary relationship to the host tick (2018)
  • Author(s): Ryo Nakao, Chihiro Sugimoto, Ken Katakura
  • Organizer: 第87回日本寄生虫学会大会
• [Presentation] Molecular identification of vector-borne and gastrointestinal protozoan parasites in domesticated goats in Myanmar (2018)
  • Author(s): Saw Bawm, Yuhei Imasato, Tay Zar Bhone Win, May June Thu, Shwe Yee Win, Lat Lat Htun, Ryo Nakao, Ken Katakura
  • Organizer: 第87回日本寄生虫学会大会
• [Presentation] Whole genome analysis of Leptospira, candidate of sp. nov. isolated from soil (2018)
  • Author(s): Toshiyuki Masuzawa, Ryo Nakao, Keiko Sakakibara, Mitsumasa Saito, Junko Tomita, Yoshiaki Kawamura
  • Organizer: 第91回日本細菌学会総会
• [Presentation] マダニとその共生微生物のハンティング (2018)
  • Author(s): 中尾 亮
  • Organizer: 第70回日本衛生動物学会大会2
  • Invited
• [Presentation] ミトゲノムによる国内マダニ種の分子系統解析 (2018)
  • Author(s): 中尾 亮
  • Organizer: 第26回ダニと疾患のインターフェイスに関するセミナー(SADI)
• [Presentation] Complete mitochondrial genome analysis of ticks (2018)
  • Author(s): Ryo Nakao, Gohta Kinoshita, Ayumi Saito, Yongjin Qiu, Keita Matsuno, Chihiro Sugimoto, Ken Katakura
  • Organizer: 14th International Congress of Parasitology (ICOPA 2018)
  • Int'l Joint Research
• [Presentation] マダニの共生微生物から探る病原体進化 (2018)
  • Author(s): 中尾 亮
  • Organizer: 第161回日本獣医学会学術集会
  • Invited
• [Presentation] マダニ内在性ウイルスの外来性微生物感染への影響の解析 (2018)
  • Author(s): 中釜尚人、平野 港、武藤芽未、西山祥子、中尾 亮、小林進太郎、苅和宏明、好井健太朗
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] Isolation of endosymbionts of ticks in Japan (2018)
  • Author(s): May June Thu, Yongjin Qiu, Chihiro Sugimoto, Ken Katakura, Norikazu Isoda, Ryo Nakao
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] 北海道の反芻動物より分離されたListeria monocytogenes の分子遺伝学的解析 (2018)
  • Author(s): 長谷部 理絵, 中尾 亮, 鈴木 章夫, 山﨑 剛士, 堀内 基広
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] Detection of two types of Strongylidea parasites from Asian elephants in Myanmar (2018)
  • Author(s): Hla Myet Chel, May June Thu, Lat Lat Htun, Saw Bawm, Ryo Nakao, Ken Katakura
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] 単為生殖型肝蛭の核DNAの遺伝子型をqPCRにより識別する方法の確立 (2018)
  • Author(s): 田渋 敦士、林 慶、中尾 稔、Uday Kumar Mohanta、福本 晋也、中尾 亮、関 まどか
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] PCR detection and phylogenetic analysis of haemoprotozoan parasites in livestock in Myanmar by sequence analysis of selected target genes (2018)
  • Author(s): Ken Katakura, Saw Bawm, Hla Myet Chel, May June Thu, Lat Lat Htun, Yuhei Imasato, Ryo Nakao
  • Organizer: 14th International Conference on Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases (MEEGID XIV)
  • Int'l Joint Research
• [Presentation] Molecular detection and genetic diversity of canine Babesia parasites from Myanmar (2017)
  • Author(s): Bawm S, June TM, Htun LL, Win MM, Nakao R, Katakura K
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] Discovery of genetically diverse phleboviruses in ticks in Zambia (2017)
  • Author(s): Kajihara M, Simuunza M, Nakao R, Mori A, Qiu Y, Eto Y, Matsuno K, Furumoto H, Ndebe J, Kapila P, Dautu G, Mweene A, Takada A
  • Organizer: 9th Tick and Tick-borne Pathogen Conference (TPP9)
  • Int'l Joint Research
• [Presentation] Serological and molecular epidemiology of Crimean-Congo hemorrhagic fever in Zambia (2017)
  • Author(s): Simuunza M, Kajihara M, Mori A, Dautu G, Qiu Y, Nakao R, Eto Y, Saasa N, Furumoto H, Fukushi S, Morikawa S, Saijo M, Yoshimatsu K, Mweene A, Takada A, Arikawa J
  • Organizer: 9th Tick and Tick-borne Pathogen Conference (TPP9)
  • Int'l Joint Research
• [Presentation] Investigation of tick-borne bacterial and protozoan pathogens in domestic dogs, cattle, and ticks in Zambia (2017)
  • Author(s): Qiu Y, Kaneko C, Kajihara M, Simuunza M, Saasa N, Furumoto H, Ndebe J, Kapila P, Thu MJ, Hang’ombe MB, Takada A, Sugimoto C, Nakao R
  • Organizer: 9th Tick and Tick-borne Pathogen Conference (TPP9)
  • Int'l Joint Research
• [Presentation] Genetic diversity of Rickettsia spp. in ticks collected from Japan (2017)
  • Author(s): Thu MJ, Qiu Y, Sugimoto C, Isoda N, Nakao R
  • Organizer: 9th Tick and Tick-borne Pathogen Conference (TPP9)
  • Int'l Joint Research
• [Presentation] Coxiella-like endosymbiont as an evolutionary marker of ticks (2017)
  • Author(s): Nakao R, Saito A, Thu MJ, Qiu Y, Matsuno K, Sugimoto C, Katakura K
  • Organizer: 9th Tick and Tick-borne Pathogen Conference (TPP9)
  • Int'l Joint Research
• [Presentation] Preliminary study on the development of a model for the complete life cycle of Babesia ovata without the need for cattle host (2017)
  • Author(s): Hatta T, Maeda H, Tsubokawa D, Mikami F, Alim MA, Matsubayashi M, Umemiya-Shirafuji R, Nakao R, Tsuji N
  • Organizer: 9th Tick and Tick-borne Pathogen Conference (TPP9)
  • Int'l Joint Research
• [Presentation] Potential of cell-free DNA as a novel diagnostic biomarker for parasite infections in dog (2017)
  • Author(s): Akter S, Nakao R, Katakura K
  • Organizer: 26th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP)
  • Int'l Joint Research
• [Presentation] ミトゲノム解析によるマダニ系統分類法の開発 (2017)
  • Author(s): 中尾 亮、木下豪太、齋藤 歩、邱 永晋、松野啓太、杉本千尋、片倉 賢
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] マダニから分離されたスピロプラズマの全ゲノム解析 (2017)
  • Author(s): 柿阪圭太、中尾 亮、Thu MJ、邱 永晋、杉本千尋、片倉 賢
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] Piroplasm spp. in deer: The 1st detection of Theileria capreoli and a novel Babesia sp. in Hokkaido (2017)
  • Author(s): Moustafa M, Elbaz E, Lee K, Mohamed W, Nakao R, Shimozuru M, Sashika M, Tsubota T
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] Discoveries of novel viruses in ticks and fruit bats by using FLDS method and MinION sequencer (2017)
  • Author(s): Matsuno K, Kajihara M, Urayama S, Nakao R, Hirai M, Qiu Y, Nunoura T, Mweene A, Hang'ombe BM, Simuunza M, Okamatsu M, Sakoda Y, Sawa H, Takada A
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] Discovery of diverse arthropod bunyaviruses in field-collected mosquitoes and ticks and their evolution (2017)
  • Author(s): Sawa H, Matsuno K, Nakao R, Sasaki M, Orba Y
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] Detection of Cryptosporidium parasite in ruminants from highland and lowland areas of Myanmar (2017)
  • Author(s): Bawm S, Moe K, Soe BK, Chel HM, Htun LL, Nakao R, Katakura K
  • Organizer: 2017 8th International Conference on Agriculture and Animal Science (ICAAS 2017)
  • Int'l Joint Research
• [Presentation] マダニが持つ微生物の多様性：共生体から病原体まで (2017)
  • Author(s): 中尾 亮
  • Organizer: 第17回北大若手研究者交流会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Invited
• [Presentation] ペプチド核酸を用いたマダニ保有原生生物叢解析法の開発 (2016)
  • Author(s): 中尾 亮、木下豪太、邱 永晋、杉本千尋、片倉 賢
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学生物資源科学部（神奈川県藤沢市）
  • Year and Date: 2016-09-06
• [Presentation] マダニとそれに共生する Coxiella 属細菌の共進化機構の考察 -両者の遺伝的多様性と系統学的解析より- (2016)
  • Author(s): 齋藤 歩、中尾 亮、Thu MJ、邱 永晋、松野啓太、杉本千尋、片倉 賢
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学生物資源科学部（神奈川県藤沢市）
  • Year and Date: 2016-09-06
• [Presentation] Genomic characterizes of Japanese spotted fever group rickettsiae in ixodid ticks (2016)
  • Author(s): Thu MJ, Qiu Y, Sugimoto C, Isoda N, Nakao R
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学生物資源科学部（神奈川県藤沢市）
  • Year and Date: 2016-09-06
• [Presentation] First isolation of Chlamydiae from an ixodid tick collected in Japan (2016)
  • Author(s): Nakao R, Abe T, Qiu Y, Thu MJ, Sugimoto C
  • Organizer: 16th International Symposium on Microbial Ecology
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] マダニ保有共生微生物群の多様性 (2016)
  • Author(s): 中尾 亮
  • Organizer: 第24回SADI（Seminar on Acari-Diseases Interface）
  • Place of Presentation: 休暇村指宿 (鹿児島県指宿市）
  • Year and Date: 2016-05-27
• [Presentation] 超高速遺伝子解析技術によるマダニ保有微生物の探索 (2016)
  • Author(s): 中尾 亮、邱 永晋、木下豪太、May June Thu、阿部貴志、片倉 賢、杉本千尋
  • Organizer: 第85回 寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ
  • Year and Date: 2016-03-19
• [Presentation] 網羅的遺伝子解析技術がもたらすマダニ研究の新展開 (2015)
  • Author(s): 中尾 亮
  • Organizer: 第23回 SADI (Seminar on Acari-Diseases Interface)
  • Place of Presentation: 名取市文化会館小ホール
  • Year and Date: 2015-06-26
• [Remarks] 北海道大学大学院獣医学研究院・獣医学部寄生虫学教室HP
  • URL: https://www.vetmed.hokudai.ac.jp/organization/parasitol/index.html
• [Remarks]
  • URL: https://www.vetmed.hokudai.ac.jp/organization/parasitol/index.html