Identification of regeneration associated gene of central nervous system: axon regeneration of corticospinal tract

• Project/Area Number: 15H05989
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Hokkaido University
• Principal Investigator: KADOYA KEN 北海道大学, 医学研究科, 特任研究助教 (30374276)
• Research Collaborator: Gibbs Daniel University of California, Dept. of Neurosciences ; Tuszynski Mark University of California, Dept. of Neurosciences ; Stobl Hans University of California, Dept. of Neurosciences ; ENDO Takeshi 北海道大学, 医学研究科, 大学院生
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 軸索 / 脊髄損傷 / 再生 / 軸索再生 / 神経幹細胞 / 移植 / cJun / 再生医学 / 神経科学

Outline of Final Research Achievements

Regeneration associated genes are identified in peripheral but not central nervous system. The purpose of the current study is to determine whether transcription factor cJun is the regeneration associated gene of central nervous system. cJun expressions were manipulated in the experimental model, in which corticospinal tract axons regenerated into grafted cells. Regulation of cJun did not affect the extent of regeneration of corticospinal axons at all, indicating that cJun is not the regeneration associated gene of corticospinal axons and that the central nervous system does not share the mechanism of regeneration with peripheral nervous system.

Research Products

• [Int'l Joint Research] University of California, San Diego(米国)
• [Int'l Joint Research] カリフォルニア大学サンディエゴ校(米国)
• [Journal Article] Spinal cord reconstitution with homologous neural grafts enables robust corticospinal regeneration (2016)
  • Author(s): Kadoya K, Lu P, Nguyen K, Lee-Kubli C, Kumamaru H, Yao L, Knackert J, Poplawski G, Dulin JN, Strobl H, Takashima Y, Biane J, Conner J, Zhang SC, Tuszynski MH
  • Journal Title: Nature Medicine Volume: 22(5) Issue: 5 Pages: 479-487
  • DOI: 10.1038/nm.4066
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 脊髄損傷の実験モデルについて (2017)
  • Author(s): 角家健
  • Organizer: 第６回脊髄再生と疼痛を語る夕べ
  • Place of Presentation: ホテルニューオータニ（東京都千代田区）
  • Invited
• [Presentation] Axon Regeneration by Homologous Tissue Reconstitution (2016)
  • Author(s): Kadoya K
  • Organizer: the 17th RIES-Hokudai International symposium
  • Place of Presentation: ガトーキングダム札幌（北海道札幌市）
  • Int'l Joint Research / Invited
• [Presentation] 神経幹細胞移植による脊髄損傷治療 (2016)
  • Author(s): 角家健
  • Organizer: 第３１回日本整形外科学会基礎学術集会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Invited