Identification of genes related to signals from transformed cells

• Project/Area Number: 15H05990
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Hokkaido University
• Principal Investigator: Maruyama Takeshi 北海道大学, 遺伝子病制御研究所, 助教 (30613872)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 哺乳類細胞競合 / 細胞死 / がん / 細胞競合

Outline of Final Research Achievements

Transformed cells surrounded by normal cells are eliminated from epithelium. Since the elimination does not occur under the condition of single culture, there should be some cross-talk signals between normal and transformed cells. From microarray analyses, we identified several genes that is up-regulated inside of normal cells. Prostaglandins were the downstream of a gene identified in the microarray analyses. Furthermore, a prostaglandin attenuated the elimination efficiency of transformed cells, indicating that signals from normal cells are also important to regulate the elimination of transformed cells.

Research Products

• [Journal Article] Rab5-regulated endocytosis plays a crucial role in apical extrusion of transformed cells (2017)
  • Author(s): Maruyama T, Saitoh S, Yako Y, Kajita M, Fujioka Y, Ohba Y, Kasai N, Sugama N, Kon S, Ishikawa S, Hayashi T, Yamazaki T, Tada M and Fujita Y
  • Journal Title: Proc Natl Acad Sci USA. Volume: 114 Issue: 12 Pages: 3157-3162
  • DOI: 10.1073/pnas.1701746114
  • NAID: 120006346108
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant