Expression and characterization of cellobiose dehydrogenases from the ascomycete fungus, Magnaporthe oryzae for management of the rice blast disease
| Project/Area Number |
15H06055
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Akita University |
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Principal Investigator |
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | いもち病菌 / セロビオース脱水素酵素 / 生物無機化学 / 構造生物学 / フラボヘムタンパク質 / 生物構造化学 / いもち病 |
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| Outline of Final Research Achievements |
Rice blast disease caused by the ascomycete fungus Magnaporthe oryzae is a serious and widespread disease of rice. The general fungicides inhibit either intracellular biosynthesis or metabolic pathway, would pose a high risk for development of fungicide resistance. Here, we have overexpressed and investigated two cellobiose dehydrogenase (CDH) homologues in M. oryzae to development a method to inhibit secreted proteins involved in the extracellular metabolic pathway for control of the fungal rice pathogen. Plant disease fungi produce and secrete hydrolases as well as oxidoreductases such as CDH to degrade the plant cell wall and to utilize cellulose as the nutrient carbon.
BLAST search using the M. oryzae genome database uncovered the existence of two CDH homologous gene, MGG_13809 (MoCDH1) and MGG_11036 (MoCDH2). The two CDH homologues were expressed in the methylotrophic yeast Pichia pastoris. MoCDH2 shows higher cellobiose dehydrogenase activity than MoCDH1.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Photoinitiated reactivity of a thiolate-ligated, Spin-crossover nonheme {FeNO}(7) complex with dioxygen2016
Author(s)
McQuilken AC, Matsumura H, Durr M, Confer AM, Sheckelton JP, Siegler MA, McQueen TM, Ivanovic-Burmazovic I, Moenne-Loccoz P, Goldberg DP
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Journal Title
J Am Chem Soc
Volume: 138
Issue: 9
Pages: 3107-3117
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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