Creation of an artificial hydrogenase enzyme with high activity using in vitro metal cofactor biosynthesis
| Project/Area Number |
15H06085
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Bio-related chemistry
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| Research Institution | Saitama University |
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Principal Investigator |
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ヒドロゲナーゼ / 鉄 / コファクター / 生合成 / 酵素 / 水素 |
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| Outline of Final Research Achievements |
[FeFe]-hydrogenase is an attractive metalloenzyme, because it can use molecular hydrogen, which is an eco-friendly energy carrier. To increase its activity, the metal cofactor as the active site was modified with artificial metal ions using apo-[FeFe]-hydrogenase and its three biosynthetic enzymes. As a result, the hydrogenases activity was not increased, whereas the other metal cofactor, iron-sulfur cluster might be decomposed. Therefore, for further study, iron-sulfur cluster biosynthetic proteins should be also mixed in the reaction mixture in order to avoid decomposition of the iron-sulfur cluster toward increasing the hydrogenase activity.
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Report
(3 results)
Research Products
(2 results)
