Kinetic analysis of the activation process of receptor tyrosine kinases by using DNA aptamer
| Project/Area Number |
15H06136
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Bio-related chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Ueki Ryosuke 東京大学, 大学院工学系研究科(工学部), 助教 (90755703)
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | DNAアプタマー / チロシンキナーゼ受容体 / アプタマー / 受容体 / 核酸 / 増殖因子 / 受容体型チロシンキナーゼ / 核酸アプタマー |
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| Outline of Final Research Achievements |
Receptor tyrosine kinases (RTKs) are known as receptors for growth factors. In this research, we tried to develop novel molecular tools that can evaluate clustering behavior of RTKs on cell surface in order to get a deeper insight into the activation mechanism of RTKs at a molecular level. We designed some DNA aptamer-based functional ligands and applied them in regulation of clustering behavior of RTKs. For analysis of RTK clustering, we focused on super-resolution imaging methods. We constructed some plasmid vectors and tested them in transfection experiments for the imaging analysis.
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Report
(3 results)
Research Products
(5 results)
