| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Outline of Final Research Achievements |
We have previously shown that Rad54B, by interacting with various proteins, forms a network that links among the cellular functions. In clinical cancer samples, constitutive upregulation of Rad54B is frequently observed and associated with poor prognosis, suggesting that the Rad54B-mediated cellular function network is involved in the neoplastic process. In this study, we aimed to reveal how Rad54B expression is controlled, and found that the Rad54B expression is regulated in a cell cycle-dependent manner. Mechanistically, the E2F family, a set of transcription factors for cell cycle-regulated genes, binds to the Rad54B promoter region to upregulate the Rad54B expression in S/G2 phase and to downregulate that in G0/G1 phase. Taken together with our previous findings that Rad54B regulates the cell cycle machinery, these data suggest the existence of a mutual regulatory mechanism between the Rad54B-mediated cellular function network and cell cycle machinery.
|
