Analysis of acid-base regulation of the cochlea toward overcoming of the inner ear disease

• Project/Area Number: 15H06224
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Niigata University
• Principal Investigator: HIGUCHI Taiga 新潟大学, 医歯学系, 助教 (60757532)
• Research Collaborator: HIBINO Hiroshi ; NAGAMORI Shushi ; UETSUKA Satoru ; NIN Fumiaki ; OGATA Genki
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: pH制御性膜タンパク質 / 内耳 / 蝸牛 / イオン電極法 / 質量分析 / 血管条 / pH制御性タンパク質

Outline of Final Research Achievements

The unique ionic environment of endolymph is produced by various ion pumps and transporters in the stria vascularis and spiral ligament. However, acid-base regulation of the inner ear is unclear including the treatment of H+ arose from the pumps to maintain the endolymph. In the present study, (1) 20 pH regulatory membrane proteins were found in the stria vascularis by LC-MS/MS. (2) The in vivo measurement system to monitor the endolymph pH was established in order to investigate the involvement of the pH regulatory proteins in the pH regulation of the inner ear.

Research Products

• [Presentation] Creation of novel model mice for reversible sensorineural hearing loss with optogenetics. (2016)
  • Author(s): Mitsuo P. Sato, Fumiaki Nin, Taiga Higuchi, Satoru Uetsuka, Takamasa Yoshida, Katsumi Doi, Hirohide Takebayashi, Kenji Tanaka, Hiroshi Hibino.
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター、(北海道・札幌市)
  • Year and Date: 2016-03-22
• [Presentation] 内耳血管条を標的とした光遺伝学による可逆性難聴の誘導 (2016)
  • Author(s): 佐藤 満雄、樋口 大河、任 書晃、吉田 崇正、上塚 学、土井 勝美、田中 謙二、日比野 浩
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜、(神奈川県・横浜市)
  • Year and Date: 2016-03-09
• [Presentation] Transient induction of deafness by optogenesis targeting the endocochlear potential in the inner ear. (2016)
  • Author(s): Mitsuo P. Sato, Fumiaki Nin, Taiga Higuchi, Satoru Uetsuka, Takamasa Yoshida, Masatsugu Masuda, Takahisa Watabe, Sho Kanzaki,Kaoru Ogawa, Katsumi Doi, Hirohide Takebayashi, Kenji Tanaka, Hiroshi Hibino.
  • Organizer: Association for Research in Otolaryngology 39th MidWinter Meeting
  • Place of Presentation: San Diego, California, (USA)
  • Year and Date: 2016-02-20
• [Presentation] 光遺伝学を駆使した難聴モデルマウスの作成 (2015)
  • Author(s): 佐藤 満雄、樋口 大河、任 書晃、吉田 崇正、緒方 元気、堀 かりん、上塚 学、増田 正次、渡部 高久、神崎 晶、小川 郁、土井 勝美、田中 謙二、日比野 浩
  • Organizer: 第62回中部日本生理学会
  • Place of Presentation: 富山大学五福キャンパス、(富山県・富山市)
  • Year and Date: 2015-11-13
• [Presentation] Transient induction of deafness by optogenesis targeting the endocochlear potential in the inner ear. (2015)
  • Author(s): Mitsuo P Sato, Fumiaki Nin, Taiga Higuchi, Satoru Uetsuka, Takamasa Yoshida, Sizuo Komune, Hidenori Inohara, Katsumi Doi, Hirohide Takebayashi, Kenji Tanaka, Hiroshi Hibino.
  • Organizer: Inner ear biology 2015
  • Place of Presentation: Roma. (Italy)
  • Year and Date: 2015-09-12
• [Presentation] PKD2L1カチオンチャネル調節機構の分子構造基盤 (2015)
  • Author(s): 樋口 大河、清水 貴浩、藤井 拓人、Nilius Bernd、酒井 秀紀
  • Organizer: 第17回応用薬理シンポジウム
  • Place of Presentation: 新潟大学医学部 有壬記念館、(新潟県・新潟市)
  • Year and Date: 2015-09-04
• [Presentation] 光遺伝学を駆使した難聴モデルマウスの作成 (2015)
  • Author(s): 佐藤 満雄、樋口 大河、任 書晃、吉田 崇正、緒方 元気、堀 かりん、上塚 学、竹林 浩秀、土井 勝美、田中 謙二、日比野 浩
  • Organizer: 第17回応用薬理シンポジウム
  • Place of Presentation: 新潟大学医学部 有壬記念館、(新潟県・新潟市)
  • Year and Date: 2015-09-04