Studies on acyltransferases that create the heterogeneity of biological membrane in bacteria
| Project/Area Number |
15H06328
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
Ogawa Takuya 京都大学, 化学研究所, 助教 (40756318)
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | リゾホスファチジン酸アシル基転移酵素 / 低温適応 / 脂質代謝 / PlsC / 膜タンパク質 / リン脂質代謝 / アシル基転移酵素 / 細胞膜生合成 / リン脂質アシル基転移酵素 / グリセロリン脂質 / 色素 |
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| Outline of Final Research Achievements |
I analyzed the functions of five homologs (SlPlsC1-SlPlsC5) of 1-acyl-sn-glycerol-3-phosphate acyltransferase (PlsC) from the psychrotrophic bacterium Shewanella livingstonensis Ac10. 1) I established the methods of purification and enzymatic assay for SlPlsC1 and revealed its enzymatic characteristics in vitro. 2) It was indicated that SlPlsC3 is involved in the biosynthesis of a yellow pigment of which the chemical structure is as yet unidentified, suggesting a novel function of PlsC-family enzymes. In addition, 3) aiming at the crystal structure analysis of PlsC, I purified a thermostable PlsC from the thermophilic bacterium Thermus thermophilus and revealed its enzymatic characteristics.
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Report
(3 results)
Research Products
(10 results)
