| Project/Area Number |
15H06365
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
Takayama Kazuo 大阪大学, 薬学研究科, 特任助教(常勤) (10759509)
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 肝細胞 / ヒトES/iPS細胞 / 薬物代謝 / CYP / 肝毒性 / 創薬試験 / CYP3A4 / ゲノム編集 / 薬物代謝酵素 / 相同組換え |
|---|
| Outline of Final Research Achievements |
In this study, we aimed to establish an efficient differentiation method for highly functioning hepatocyte-like cells from human ES/iPS cells. First, we attempted to improve the hepatocyte differentiation method. Next, we developed a method to concentrate the highly functioning hepatocyte-like cells. The hepatic functions of human ES/iPS cell-derived hepatocyte-like cells were enhanced by culturing with conditioned medium of hepatocytes and cholangiocytes. In future, our group is going to establish the genetically engineered human ES/iPS cells, which carry puromycin resistant cassette under the CYP3A4.
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