| Project/Area Number |
15H06449
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Prosthodontics/ Dental materials science and
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | microRNA / 炎症性刺激 / 細胞増殖 / MC3T3-E1細胞 / miR-146a / 骨芽細胞 / TRAF6 / IRAK１ / micro RNA / ストレス応答 / messenger RNA / バイオマーカー / TNF-α / 持続的圧縮応力 |
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| Outline of Final Research Achievements |
In the present study, we attempted to identified miRNAs in response to TNF-α stimulation or compressive force and to analyze those functions in MC3T3-E1 cells.
Microarray analysis followed by reverse transcription-quantitative polymerase chain reaction revealed that TNF-α stimulation or compressive force at 294 Pa for 24 h in MC3T3-E1 cells increased levels of miR-146a-5p. Overexpression of miR-146a-5p in MC3T3-E1 showed effects on early stage of cell proliferation. Furthermore, in silico analysis predicted TRAF6 and IRAK1 as target genes of miR-146a-5p. However, TRAF6 and IRAK1 mRNA levels did not alter in MC3T3-E1 cell overexpressing miR-146a-5p gene and did not affect cell proliferation.
In conclusion, TNF-α stimulation and compressive force affected expressions of miR-146a-5p in MC3T3-E1 cells. In addition, miR-146a-5p affects early stage of cell proliferation. Further studies to investigate the target genes and functions of miR-146a-5p in MT3T3-E1 cells will be required.
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