The periodontal regeneration therapy with novel amelogenin binding protein Grp78
| Project/Area Number |
15H06492
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Periodontology
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-08-28 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | アメロジェニン / Grp78 |
|---|
| Outline of Final Research Achievements |
Our recent proteomic study identified glucose-regulated protein 78 (Grp78) as a new amelogenin-binding protein. we demonstrate the cellular responses induced by the biological interaction between amelogenin and Grp78 in the human undifferentiated PDL cell line 1-17, which possesses the most typical characteristics of PDLSCs. Microarray analysis indicated that rM180 and Grp78 regulate the expression profiles of cell migration-associated genes in 1-17 cells. Moreover, Grp78 overexpression enhanced rM180-induced cell migration and adhesion without affecting cell proliferation, while silencing of Grp78 diminished these activities. Finally, binding of rM180 to Grp78 promoted the formation of lamellipodia, and the simultaneous activation of Rac1 was also demonstrated by NSC23766, a widely accepted Rac1 inhibitor. These results suggest that Grp78 is essential for enhancing amelogenin-induced migration in 1-17 cells.
|
Report
(3 results)
Research Products
(14 results)
-
[Journal Article] Grp78 is critical for amelogenin-induced cell migration in a multipotent clonal human periodontal ligament cell line.2016
Author(s)
Toyoda K, Fukuda T, Sanui T*, Tanaka U, Yamamichi K, Atomura R, Maeda H, Tomokiyo A, Taketomi T, Uchiumi T, Nishimura F
-
Journal Title
Journal of Cellular Physiology
Volume: 231
Issue: 2
Pages: 414-427
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-
-
[Presentation] Amelogenin Modulates Macrophage Phenotype During Inflammatory Responses.2016
Author(s)
Kensuke Yamamichi, Hajime Akiyama, Takao Fukuda, Terukazu Sanui, Kyosuke Toyoda, Urara Tanaka, Takaharu Taketomi, Fusanori Nishimura
Organizer
4th General Session & Exhibition of the International Association for Dental Research
Place of Presentation
COEX Convention ＆ Exbition Center Seoul, Republic of Korea
Year and Date
2016-06-22
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
