The effects of the bone quality on bones regenerated by LIPUS

• Project/Area Number: 15H06646
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Nihon University
• Principal Investigator: MANAKA Soichiro 日本大学, 歯学部, 専修医 (00754954)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 低出力超音波（LIPUS） / 骨質 / コラーゲン（collagen） / MMP / TIMP / コラーゲン / 架橋構造 / AGE

Outline of Final Research Achievements

In this study, when osteoblasts were given low-intensity pulsed ultrasound stimulation used as a mechanical stress, we aimed to clarify the effect of LIPUS stimulation on collagen metabolism in osteoblastic bone formation from cell biology and molecular biology.
Our results demonstrated that LIPUS induced the gene and protein expression of type I collagen, whereas it reduced MMP-1, 3 and 13. In addition, LIPUS didn't affect the both expression of MMP-2 and TIMPs. These results suggest that LIPUS promotes osteogenesis through the ATP-P2X7 receptors in osteoblasts during collagen synthesis.

Research Products

• [Presentation] P2X7 receptor mediates collagen synthesis induced by LIPUS (2016)
  • Author(s): Soichiro Manaka, Natsuko Tanabe, Mayu Nagao, Masako Naito, Kenji Sugai, Noboru Shiomi, Naoto Suzuki, Shuichi Sato
  • Organizer: 102th Meeting of the American Academy of Periodontology (International Academic Societies)
  • Place of Presentation: San Diego convention center (U.S.A, California)
  • Year and Date: 2016-09-13
  • Int'l Joint Research
• [Presentation] LIPUS刺激は骨芽細胞のATP-P2X7受容体を介してコラーゲン代謝を促進する (2016)
  • Author(s): 間中総一郎, 田邉奈津子, 長尾麻由, 鈴木直人, 前野正夫, 佐藤秀一
  • Organizer: 第25回硬組織再生生物学会学術大会・総会
  • Place of Presentation: 日本大学歯学部（東京都千代田区）
  • Year and Date: 2016-08-19
• [Presentation] 低出力超音波刺激はP2X7受容体を介して骨芽細胞のコラーゲン代謝を促進する (2016)
  • Author(s): 間中総一郎, 田邉奈津子, 長尾麻由, 髙山忠裕, 仙田直樹, 平野治朗, 鈴木直人, 佐藤秀一
  • Organizer: 第59回春季日本歯周病学会学術大会
  • Place of Presentation: 鹿児島文化センター (鹿児島県鹿児島市)
  • Year and Date: 2016-05-20
• [Presentation] Low-intensity pulsed ultrasound promotes osteogenesis through the P2X7 receptor in osteoblasts during collagen synthesis. (2016)
  • Author(s): Soichiro Manaka, Natsuko Tanabe, Mayu Nagao, Manami Ozaki, Masao Maeno, Naoto Suzuki, Shuichi Sato
  • Organizer: 45th Annual Meeting & Exhibition of the American Association for Dental Research (International Academic Societies)
  • Place of Presentation: Los Angeles convention center (U.S.A, California)
  • Year and Date: 2016-03-16
  • Int'l Joint Research