| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Mate-choice experiments of male Medaka were conducted between two skin-color variants , color interfere (ci) and Actb-SLα:GFP (soma), which are different in the skin color regulated by a single gene. These strains were reared with various tank mates from hatch until sexual maturation. Matured males were allowed to mate with novel two females of different strains for 30 minutes, and the number of courtship behaviors to each female was counted to assess his preference. Preference test with pair of two strains female was repeated for four successive days. When these strains had been reared separately, the males strongly preferred females of the same strain. However, if the strains had been reared in a mixed condition or each fish was reared individually, the males increased interests in females of the other strain. These results indicate that sexual preferences of males are established being affected by fish they grow up with.
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