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Application of probiotics for the prevention of bacterial infection after operation in dental field

Research Project

Project/Area Number 15H06700
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionTsurumi University

Principal Investigator

SOGABE Kaoru  鶴見大学, 歯学部, 学部助手 (40758489)

Project Period (FY) 2015-08-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsプロバイオティクス / バクテリオシン様物質 / Lactobacillus crispatus / Streptococcus pneumoniae / 歯学 / 術後感染
Outline of Final Research Achievements

Lactobacillus crispatus LBS 17-11 was isolated from an oral cavity of human subject, and possess growth inhibition activity against Streptococcus pneumoniae and Haemophilus influenzae. The objective of this study was to purify a bacteriocin-like substance, and to determine its characteristic properties. Antibacterial activities against oral bacteria were determined by a radial diffusion assay. Purification procedure for bacteriocin-like substance was achieved with ammonium sulfate precipitation of culture supernatant, and subsequent extraction with chloroform. Antibacterial substance was partially purified up to 2045-fold specific activity (unit/mg protein). The inhibitory activity was shown against S. pneumoniae, but Gram negative periodontopathic bacteria. The antimicrobial activity was heat stable and partially lost by the treatment with trypsin and proteinase K. The molecular mass of bacteriocin-like substance was estimated to be approximately 7 kDa based on SDS-PAGE.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • Research Products

    (10 results)

All 2017 2016 2015

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Comparison of Salivary Hemoglobin Measurements for Periodontitis Screening2017

    • Author(s)
      Ayako Okada, Yoshiaki Nomura, Karo Sogabe et al.
    • Journal Title

      Journal of Oral Science

      Volume: 59 Pages: 63-69

    • NAID

      130005519762

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 歯周病を対象とした唾液生化学検査に対する受診者の意識調査2017

    • Author(s)
      西辻直之,古藤真実,福澤洋一,矢吹義秀,上谷公之,久保宏史,吉野浩和,長井博昭,中曽根隆一,矢島正隆,岡田彩子,有吉芽生,曽我部薫,菊地朋宏,宮之原真由,山田秀則,村田貴俊,野村義明,花田信弘.
    • Journal Title

      日本口腔衛生学会雑誌

      Volume: 67 Pages: 89-93

    • NAID

      130005696988

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the Sampling Specimens for the Evaluation of Periodontal Bacteria2017

    • Author(s)
      Ayako Okada, Kaoru Sogabe, Hiroaki Takeuchi, Masaaki Okamoto, Yoshiaki Nomura, Nobuhiro Hanada
    • Journal Title

      Journal of Oral Science

      Volume: in press

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization and purification of a bacteriocin-like substance produced by Lactobacillus crispatus LBS 17-11 isolated from an oral cavity of human subject2017

    • Author(s)
      Gaku Akimaru, Susumu Imai, Kaoru Sogabe, Ayako Okada,  Tomohiko Terai, Takekazu Okumura, Nobuhiro Hanada, Hiroshi Kawahara
    • Journal Title

      Asian Pacific Journal of Dentistry

      Volume: 17 Pages: 9-14

    • NAID

      40021199033

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 全身麻酔下歯科口腔外科手術後に角膜障害をきたした2症例2016

    • Author(s)
      中村泰輔、山本徹、舘野健、鈴木将之、福田瑠美、曽我部薫、堂下幹二、阿部佳子、河原博
    • Journal Title

      日本歯科麻酔学会雑誌

      Volume: 4 Pages: 26-28

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Opportunistic Bacteria in Tonsil and Dental Plaque are Indicator for Oral Care2016

    • Author(s)
      Chiyoko Hakuta, Ayako Okada, Kaoru Sogabe, Erika Kakuta, Keiko Endo, Susumu Imai, Masaaki Okamoto, Yoshiaki Nomura and Nobuhiro Hanada
    • Journal Title

      International Journal of Oral and Craniofacial Science

      Volume: 2 Pages: 30-34

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Haemophilus influenzae に対するプロバイオティクス候補菌Lactobacillus crispatusの効果2017

    • Author(s)
      曽我部薫、岡田彩子、今井奬、花田信弘.
    • Organizer
      第6回口腔保健用機能性食品研究会・総会
    • Place of Presentation
      鶴見大学会館(神奈川県横浜市)
    • Year and Date
      2017-01-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] 3DSトレ-を用いた次亜塩素酸電解水の臨床的評価:第2報2016

    • Author(s)
      岡田彩子, 村田貴俊, マティン カイル-ル, 宮之原真由, 曽我部 薫, 大塚良子, 須藤智省, 松澤匡純, 桐原 大, 花田信弘
    • Organizer
      第17回日本口腔機能水学会学術大会
    • Place of Presentation
      梅田センタービル(大阪府,大阪市)
    • Year and Date
      2016-03-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] エナメル上皮腫に対し下顎骨辺縁切除および腸骨海綿骨による再建術を行った褐色細胞腫既往患者の周術期管理2015

    • Author(s)
      福田瑠美、曽我部薫、鈴木将之、市川由香、日高亨彦、中村泰輔、山本徹、阿部佳子、河原博
    • Organizer
      第43回日本歯科麻酔学会総会・学術集会
    • Place of Presentation
      東京医科歯科大学(東京都,文京区)
    • Year and Date
      2015-10-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] Trial of a Liquid Carrier Type Dental Drug Delivery Tray2015

    • Author(s)
      A. OKADA, T. MURATA, K. MATIN, M. MIYANOHARA, K. SOGABE, R. OTSUKA,M. KIRIHARA, M. MATSUZAWA, T. SUDO, S. IMAI, J. TAGAMI and N. HANADA
    • Organizer
      第63回国際歯科研究学会 日本部会・学術大会
    • Place of Presentation
      福岡国際会議場(福岡県,福岡市)
    • Year and Date
      2015-10-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2015-08-26   Modified: 2018-03-22  

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