Application of probiotics for the prevention of bacterial infection after operation in dental field

• Project/Area Number: 15H06700
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Tsurumi University
• Principal Investigator: SOGABE Kaoru 鶴見大学, 歯学部, 学部助手 (40758489)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: プロバイオティクス / バクテリオシン様物質 / Lactobacillus crispatus / Streptococcus pneumoniae / 歯学 / 術後感染

Outline of Final Research Achievements

Lactobacillus crispatus LBS 17-11 was isolated from an oral cavity of human subject, and possess growth inhibition activity against Streptococcus pneumoniae and Haemophilus influenzae. The objective of this study was to purify a bacteriocin-like substance, and to determine its characteristic properties. Antibacterial activities against oral bacteria were determined by a radial diffusion assay. Purification procedure for bacteriocin-like substance was achieved with ammonium sulfate precipitation of culture supernatant, and subsequent extraction with chloroform. Antibacterial substance was partially purified up to 2045-fold specific activity (unit/mg protein). The inhibitory activity was shown against S. pneumoniae, but Gram negative periodontopathic bacteria. The antimicrobial activity was heat stable and partially lost by the treatment with trypsin and proteinase K. The molecular mass of bacteriocin-like substance was estimated to be approximately 7 kDa based on SDS-PAGE.

Research Products

• [Journal Article] Comparison of Salivary Hemoglobin Measurements for Periodontitis Screening (2017)
  • Author(s): Ayako Okada, Yoshiaki Nomura, Karo Sogabe et al.
  • Journal Title: Journal of Oral Science Volume: 59 Pages: 63-69
  • DOI: 10.2234/josnusd.16-0204
  • Peer Reviewed / Open Access
• [Journal Article] 歯周病を対象とした唾液生化学検査に対する受診者の意識調査 (2017)
  • Author(s): 西辻直之，古藤真実，福澤洋一，矢吹義秀，上谷公之，久保宏史，吉野浩和，長井博昭，中曽根隆一，矢島正隆，岡田彩子，有吉芽生，曽我部薫，菊地朋宏，宮之原真由，山田秀則，村田貴俊，野村義明，花田信弘.
  • Journal Title: 日本口腔衛生学会雑誌 Volume: 67 Pages: 89-93
  • Peer Reviewed
• [Journal Article] Characterization of the Sampling Specimens for the Evaluation of Periodontal Bacteria (2017)
  • Author(s): Ayako Okada, Kaoru Sogabe, Hiroaki Takeuchi, Masaaki Okamoto, Yoshiaki Nomura, Nobuhiro Hanada
  • Journal Title: Journal of Oral Science Volume: in press Pages: in press
  • Peer Reviewed
• [Journal Article] Characterization and purification of a bacteriocin-like substance produced by Lactobacillus crispatus LBS 17-11 isolated from an oral cavity of human subject (2017)
  • Author(s): Gaku Akimaru, Susumu Imai, Kaoru Sogabe, Ayako Okada, Tomohiko Terai, Takekazu Okumura, Nobuhiro Hanada, Hiroshi Kawahara
  • Journal Title: Asian Pacific Journal of Dentistry Volume: 17 Pages: 9-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 全身麻酔下歯科口腔外科手術後に角膜障害をきたした2症例 (2016)
  • Author(s): 中村泰輔、山本徹、舘野健、鈴木将之、福田瑠美、曽我部薫、堂下幹二、阿部佳子、河原博
  • Journal Title: 日本歯科麻酔学会雑誌 Volume: 4 Pages: 26-28
  • Peer Reviewed
• [Journal Article] Opportunistic Bacteria in Tonsil and Dental Plaque are Indicator for Oral Care (2016)
  • Author(s): Chiyoko Hakuta, Ayako Okada, Kaoru Sogabe, Erika Kakuta, Keiko Endo, Susumu Imai, Masaaki Okamoto, Yoshiaki Nomura and Nobuhiro Hanada
  • Journal Title: International Journal of Oraｌ and Craniofacial Science Volume: 2 Pages: 30-34
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Haemophilus influenzae に対するプロバイオティクス候補菌Lactobacillus crispatusの効果 (2017)
  • Author(s): 曽我部薫、岡田彩子、今井奬、花田信弘．
  • Organizer: 第6回口腔保健用機能性食品研究会・総会
  • Place of Presentation: 鶴見大学会館（神奈川県横浜市）
  • Year and Date: 2017-01-29
• [Presentation] 3DSトレ－を用いた次亜塩素酸電解水の臨床的評価：第2報 (2016)
  • Author(s): 岡田彩子, 村田貴俊, マティン カイル－ル, 宮之原真由, 曽我部 薫, 大塚良子, 須藤智省, 松澤匡純, 桐原 大, 花田信弘
  • Organizer: 第17回日本口腔機能水学会学術大会
  • Place of Presentation: 梅田センタービル（大阪府,大阪市）
  • Year and Date: 2016-03-25 – 2016-03-26
• [Presentation] エナメル上皮腫に対し下顎骨辺縁切除および腸骨海綿骨による再建術を行った褐色細胞腫既往患者の周術期管理 (2015)
  • Author(s): 福田瑠美、曽我部薫、鈴木将之、市川由香、日高亨彦、中村泰輔、山本徹、阿部佳子、河原博
  • Organizer: 第43回日本歯科麻酔学会総会・学術集会
  • Place of Presentation: 東京医科歯科大学（東京都,文京区)
  • Year and Date: 2015-10-30 – 2015-10-31
• [Presentation] Trial of a Liquid Carrier Type Dental Drug Delivery Tray (2015)
  • Author(s): A. OKADA, T. MURATA, K. MATIN, M. MIYANOHARA, K. SOGABE, R. OTSUKA,M. KIRIHARA, M. MATSUZAWA, T. SUDO, S. IMAI, J. TAGAMI and N. HANADA
  • Organizer: 第63回国際歯科研究学会 日本部会・学術大会
  • Place of Presentation: 福岡国際会議場（福岡県,福岡市）
  • Year and Date: 2015-10-30 – 2015-10-31
  • Int'l Joint Research