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Breeding of low-allergen

Research Project

Project/Area Number 15H06740
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Science in genetics and breeding
Research InstitutionRyukoku University

Principal Investigator

Endo Takashi  龍谷大学, 農学部, 教授 (60068830)

Research Collaborator Valárik Miro  
Project Period (FY) 2015-08-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsセリアック病 / パンコムギ / 染色体欠失 / グリアジン / 免疫抗原
Outline of Final Research Achievements

Celiac disease is a serious disorder where the ingestion of gluten leads to damage in the small intestine. The major antigens of celiac disease are gamma gliadin on chromosome 1D and alpha gliadin on chromosome 6D. The purpose of this study was to create bread wheat lines that lack both parts of chromosomes 1D and 6D where the genes for gamma and alpha gliadins are located, respectively. In this study two chromosome partial deletion lines, 1DS-5 and 6DS-4 were selected to make F1 between them, and then F2 progeny was obtained. By chromosome observation and PCR analysis, one plant was confirmed to be homozygous for both 1DS-5 and 6DS-4. This plant was propagated to establish double deletion lines lacking both gamma and alpha gliadins.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report

URL: 

Published: 2015-08-26   Modified: 2018-03-22  

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