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Development of an analytical method for trace elemental analysis in single particle/single cell

Research Project

Project/Area Number 15H06768
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Analytical chemistry
Research InstitutionKwansei Gakuin University

Principal Investigator

Iwai Takahiro  関西学院大学, 理工学部, 助教 (90756694)

Research Collaborator CHIBA Koichi  
OKINO Akitoshi  
INAGAKI Kazumi  
Project Period (FY) 2015-08-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords単一細胞分析 / 微小量試料分析 / 微量元素分析 / ヒト単一細胞分析 / 単一微粒子分析 / 環境化学 / 分析化学
Outline of Final Research Achievements

To analyze trace elements in single cell and single particle, an ICP analytical system was established by hyphenating droplet direct injection nebulizer, desolvation system and ICP spectrometer. we succeeded to increase mass signal intensity of Mg in single algae cell 30 times by optimizing sample heating region in desolvation system. To evaluate spectral analysis precision of the system, uniform-sized particles of magnetic polymer latex were analyzed and 19.5% of relative standard deviation was obtained from this system. Single human cancer cells were introduced into this system and emission spectra of Mg and Ca, which is contained in single cell pg level, were successfully detected simultaneously.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • Research Products

    (13 results)

All 2017 2016 2015

All Presentation (11 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (2 results)

  • [Presentation] High-sensitive Elemental Analysis of Single Human Cell using Droplet Injection ICP-AES/MS2017

    • Author(s)
      T. Iwai, S. Shunsuke, S. Kohno, M. Aida, K. Kakegawa, T. Miyake, H. Miyahara, Y. Matsumoto, K. Chiba, A. Okino
    • Organizer
      European Winter Conference on Plasma Spectrochemistry 2017
    • Place of Presentation
      St. Anton Am Arlberg, Austria
    • Year and Date
      2017-02-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Single Cell Elemental Analysis of Human Cells using Droplet Injection ICP-AES/MS2016

    • Author(s)
      S. Hosoda, M. Aida, K. Kakegawa, T. Iwai, H. Miyahara, K. Chiba, A. Okino
    • Organizer
      2016 SCIX FACSS
    • Place of Presentation
      Minneapolis, USA
    • Year and Date
      2016-09-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ドロプレットICP発光・質量分析装置による動物細胞の単一細胞微量元素分析2016

    • Author(s)
      細田駿介, 相田真里, 掛川賢, 岩井貴弘, 宮原秀一, 千葉光一, 沖野晃俊
    • Organizer
      日本分析化学会第65年会
    • Place of Presentation
      北海道大学(北海道,札幌市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 単一細胞分析のためのドロプレット-ICPMS用脱溶媒装置の加熱部の検討2016

    • Author(s)
      河野聡史,細田駿介, 相田真里, 掛川賢, 岩井貴弘, 宮原秀一, 千葉光一, 沖野晃俊
    • Organizer
      日本分析化学会第65年会
    • Place of Presentation
      北海道大学(北海道,札幌市)
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] ドロプレットICP-AES/MSによるヒト細胞の単一細胞微量元素分析2016

    • Author(s)
      細田駿介, 相田真里, 掛川賢, 岩井貴弘, 宮原秀一, 千葉光一, 沖野晃俊
    • Organizer
      2016筑波セミナー
    • Place of Presentation
      幕張メッセ(千葉県,千葉市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 単一細胞分析のためのドロプレット用脱溶媒装置の検討2016

    • Author(s)
      河野聡史,細田駿介, 相田真里, 掛川賢, 岩井貴弘, 宮原秀一, 千葉光一, 沖野晃俊
    • Organizer
      2016筑波セミナー
    • Place of Presentation
      幕張メッセ(千葉県,千葉市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] ドロプレット用脱溶媒装置の加熱温度が単一細胞分析に与える影響の調査2016

    • Author(s)
      細田駿介, 相田真里, 掛川賢, 岩井貴弘, 宮原秀一, 千葉光一, 沖野晃俊
    • Organizer
      日本分析化学会第76回分析化学討論会
    • Place of Presentation
      岐阜薬科大学・岐阜大学(岐阜県,岐阜市)
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] マイクロ粒子を用いた単一細胞分析用ドロプレットICP発光・質量分析装置の評価2016

    • Author(s)
      細田駿介, 相田真里, 掛川賢, 岩井貴弘, 宮原秀一, 千葉光一, 沖野晃俊
    • Organizer
      平成28年度日本分光学会年次講演会
    • Place of Presentation
      大阪大学(大阪府,豊中市)
    • Year and Date
      2016-05-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 過渡的信号のための高速信号取得法と単一細胞分析への適用2015

    • Author(s)
      岩井貴弘
    • Organizer
      プラズマ分光分析研究会 第95回講演会
    • Place of Presentation
      株式会社堀場製作所(東京都千代田区)
    • Year and Date
      2015-10-09
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ICP-AES/MSによる単一細胞分析のための擬似細胞試料の検討2015

    • Author(s)
      細田駿介, 相田真里, 掛川賢, 岩井貴弘, 宮原秀一, 千葉光一, 沖野晃俊
    • Organizer
      第76回応用物理学会秋季学術講演会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 表面付着物プラズマ質量分析装置を用いた化学剤定量分析法の開発2015

    • Author(s)
      岩井貴弘, 掛川賢, 相田真里, 長島央行, 名児耶友樹, 金森美江子, 宮原秀一, 瀬戸康雄, 沖野晃俊
    • Organizer
      日本分析化学会第64年会
    • Place of Presentation
      九州大学伊都キャンパス(福岡市西区)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Book] Microplasma Atomic Emission Spectrometry, Encyclopedia of Plasma Technology2017

    • Author(s)
      T. Iwai, H. Miyahara, A. Okino
    • Total Pages
      7
    • Publisher
      Taylor & Francis
    • Related Report
      2016 Annual Research Report
  • [Book] Plasma Spectroscopy, The Encyclopedia of Analytical Chemistry2017

    • Author(s)
      A. Okino, H. Miyahara, T. Iwai, K. Chiba
    • Total Pages
      17
    • Publisher
      John Wiley & Sons
    • Related Report
      2016 Annual Research Report

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Published: 2015-08-26   Modified: 2018-03-22  

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