Identification of transcriptional regulators of nuclear receptors
| Project/Area Number |
15H06771
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Kwansei Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 炎症 / 転写制御 / シグナル伝達 / 創薬 / プロテオーム / 薬学 / 遺伝子 / 翻訳後修飾 |
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| Outline of Final Research Achievements |
To elucidate the complex transcriptional regulatory mechanism by nuclear receptors, we tried to identify interacting molecules to nuclear receptor glucocorticoid receptor (GR). For the proteome analysis, we examined ChIP-MS method and BioID method. We employ BioID method which had appropriate expression and function of the GR construct for the proteome analysis. BioID detected a molecule interacting with GR as a biotinylate protein. Although we need to improve the experimental condition to identify the interacting molecules to GR, we develop the foundation of the experiment system to gain our knowledge of transcription by GR.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] A structure-activity relationship of non-peptide macrocyclic histone deacetylase inhibitors and their anti-proliferative and anti-inflammatory activities.2015
Author(s)
Tapadar S, Fathi S, Raji I, Omesiete W, Kornacki JR, Mwakwari SC, Miyata M, Mitsutake K, Li JD, Mrksich M, Oyelere AK.
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Journal Title
Bioorg Med Chem.
Volume: 23(24)
Pages: 7543-64
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Glucocorticoids suppress inflammation via the upregulation of negative regulator IRAK-M2015
Author(s)
Miyata M, Lee JY, Susuki-Miyata S, Wang WY, Xu H, Kai H, Kobayashi KS, Flavell RA, Li JD.
Organizer
International Symposium on Chronic Inflammatory Diseases, Kumamoto (ISCIDK2015)
Place of Presentation
Graduate School of Pharmaceutical Sciences, Kumamoto University, (熊本県, 熊本市)
Year and Date
2015-10-16
Related Report
Int'l Joint Research
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