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Identification of transcriptional regulators of nuclear receptors

Research Project

Project/Area Number 15H06771
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionKwansei Gakuin University

Principal Investigator

Miyata Masanori  関西学院大学, 理工学部, 助教 (10756670)

Project Period (FY) 2015-08-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords炎症 / 転写制御 / シグナル伝達 / 創薬 / プロテオーム / 薬学 / 遺伝子 / 翻訳後修飾
Outline of Final Research Achievements

To elucidate the complex transcriptional regulatory mechanism by nuclear receptors, we tried to identify interacting molecules to nuclear receptor glucocorticoid receptor (GR). For the proteome analysis, we examined ChIP-MS method and BioID method. We employ BioID method which had appropriate expression and function of the GR construct for the proteome analysis. BioID detected a molecule interacting with GR as a biotinylate protein. Although we need to improve the experimental condition to identify the interacting molecules to GR, we develop the foundation of the experiment system to gain our knowledge of transcription by GR.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • Research Products

    (15 results)

All 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (2 results) Remarks (2 results)

  • [Int'l Joint Research] Georgia State University(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Deubiquitinase CYLD acts as a negative regulator for bacterium NTHi-induced inflammation by suppressing K63-linked ubiquitination of MyD88.2016

    • Author(s)
      *Lee BC, *Miyata M (*contributed equally), Lim JH, Li JD.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 113(2) Issue: 2

    • DOI

      10.1073/pnas.1518615113

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nontypeable Haemophilus influenzae-Induced MyD88 Short Expression Is Regulated by Positive IKKβ and CREB Pathways and Negative ERK1/2 Pathway.2015

    • Author(s)
      Andrews CS, Miyata M, Susuki-Miyata S, Lee BC, Komatsu K, Li JD.
    • Journal Title

      PLoS One.

      Volume: 10(12) Issue: 12 Pages: e0144840-e0144840

    • DOI

      10.1371/journal.pone.0144840

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A structure-activity relationship of non-peptide macrocyclic histone deacetylase inhibitors and their anti-proliferative and anti-inflammatory activities.2015

    • Author(s)
      Tapadar S, Fathi S, Raji I, Omesiete W, Kornacki JR, Mwakwari SC, Miyata M, Mitsutake K, Li JD, Mrksich M, Oyelere AK.
    • Journal Title

      Bioorg Med Chem.

      Volume: 23(24) Pages: 7543-64

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Vinpocetine inhibits Streptococcus pneumoniae-induced upregulation of mucin MUC5AC expression via induction of MKP-1 phosphatase in the pathogenesis of otitis media.2015

    • Author(s)
      Lee JY, Komatsu K, Lee BC, Miyata M, O'Neill Bohn A, Xu H, Yan C, Li JD.
    • Journal Title

      J Immunol.

      Volume: 194(12) Issue: 12 Pages: 5990-8

    • DOI

      10.4049/jimmunol.1401489

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cross-talk between PKA-Cβ and p65 mediates synergistic induction of PDE4B by roflumilast and NTHi.2015

    • Author(s)
      Susuki-Miyata S, Miyata M, Lee BC, Xu H, Kai H, Yan C, Li JD.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 112 Issue: 14

    • DOI

      10.1073/pnas.1418716112

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Deubiquitinase CYLD acts as a negative regulator for bacterium NTHi-induced inflammation by suppressing K63-linked ubiquitination of MyD88.2016

    • Author(s)
      Miyata M, Lee BC, Lim JH, Li JD.
    • Organizer
      第89回 生化学会
    • Place of Presentation
      仙台国際センター(宮城県・仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 慢性閉塞性肺疾患 (COPD) 治療薬による相乗的分子制御機構の解明.2016

    • Author(s)
      宮田 将徳,宮田(薄)聖子,沖米田 司,Li Jian-Dong.
    • Organizer
      第89回日本薬理学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-03-10
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 慢性閉塞性肺疾患 (COPD) の治療薬に対する生体応答機構の解明.2015

    • Author(s)
      宮田 将徳,宮田(薄)聖子,沖米田 司,Li Jian-Dong.
    • Organizer
      第38回分子生物学会年会・第88回生化学会大会合同大会(BMB2015)
    • Place of Presentation
      神戸ポートアイランド (兵庫県・神戸市)
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Glucocorticoids suppress inflammation via the upregulation of negative regulator IRAK-M2015

    • Author(s)
      Miyata M, Lee JY, Susuki-Miyata S, Wang WY, Xu H, Kai H, Kobayashi KS, Flavell RA, Li JD.
    • Organizer
      International Symposium on Chronic Inflammatory Diseases, Kumamoto (ISCIDK2015)
    • Place of Presentation
      Graduate School of Pharmaceutical Sciences, Kumamoto University, (熊本県, 熊本市)
    • Year and Date
      2015-10-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A novel cross-talk between PKA-Cβ and p65 mediates synergistic induction of PDE4B by roflumilast and NTHi.2015

    • Author(s)
      Susuki-Miyata S, Miyata M, Lee BC, Xu H, Kai H, Yan C, Li JD.
    • Organizer
      International Symposium on Chronic Inflammatory Diseases, Kumamoto (ISCIDK2015)
    • Place of Presentation
      Graduate School of Pharmaceutical Sciences, Kumamoto University (熊本県,・熊本市)
    • Year and Date
      2015-10-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Book] The LUNG perspectives/COPD 治療薬(グルココルチコイド, PDE4阻害剤) の作用メカニズム2016

    • Author(s)
      宮田 将徳
    • Total Pages
      5
    • Publisher
      メディカルレビュー社
    • Related Report
      2016 Annual Research Report
  • [Book] 生化学ミニレビュー/抗炎症薬による細胞内シグナルフィードバックの制御2016

    • Author(s)
      宮田 将徳
    • Total Pages
      3
    • Publisher
      公益社団法人 日本生化学会
    • Related Report
      2016 Annual Research Report
  • [Remarks] 関西学院大学 沖米田研究室 ホームページ

    • URL

      http://sci-tech.ksc.kwansei.ac.jp/~okiyoneda/okilab.html

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Remarks] 関西学院大学 研究者データベース

    • URL

      http://researchers.kwansei.ac.jp/view?l=ja&u=200000203

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-08-26   Modified: 2018-03-22  

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