Start-up study for the promotion mechanisms of diabetic nephropathy by oral bacteria and the preventive dentistry

• Project/Area Number: 15H06802
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Social dentistry
• Research Institution: Fukuoka Dental College
• Principal Investigator: Takata Shunsuke 福岡歯科大学, 口腔歯学部, 医員 (10757826)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 糖尿病 / 腎症 / Porphyromonas gingivalis / lipopolysaccharide / toll-like receptor / 糖尿病性腎症 / 糖尿病マウス / 歯周病 / 糸球体硬化 / 終末糖化産物 / 1型コラーゲン / ストレプトゾトシン / １型糖尿病

Outline of Final Research Achievements

We found that renal glomeruli in diabetic environment express toll-like receptors (TLR), and that diabetic mice administering periodontal pathogenic substances become nephropathy but healthy mice do not become nephropathy. TLR is an immune molecule that is primarily expressed on leukocyte, which recognizes bacteria and causes inflammation. Healthy blood vessels do not normally express. In heavy periodontitis, bacteria enter easily into the blood with brushing teeth, so it is thought that in human with both diabetes and periodontitis bacteria enter into the blood and arrive at the kidney, and nephropathy occurs by combining bacteria with TLR which is expressed in diabetic glomeruli. Therefore, it was concluded that treatment and prevention of periodontal disease in human with diabetes is very important for prevention of nephropathy.

Research Products

• [Journal Article] Effects of TLR4 blocking on diabetic nephropathy promoted by the Porphyromonas gingivalis lipopolysaccharide. (2017)
  • Author(s): Kajiwara, K., Takata S., To, T., Takara, K., Hatakeyama, Y., Tamaoki, S., Darveau, R., Ishikawa, I., Sawa, Y.
  • Journal Title: Diabetology & Metabolic Syndrome Volume: 印刷中
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] The cytokine inductions in glomerular endothelial cells with Porphyromonas gingivalis lipopolysaccharide yhrough TLR2. (2016)
  • Author(s): Takata, S., Kajiwara, K., Hatakeyama, Y., Ishikawa, H., Sawa, Y.
  • Organizer: 94th International Association of Dental Research
  • Place of Presentation: Seoul
  • Year and Date: 2016-06-24
  • Int'l Joint Research
• [Presentation] The cytokine inductions in glomerular endothelial cells with Porphyromonas gingivalis lipopolysaccharide through TLR2 (2016)
  • Author(s): Takata S, Kajiwara K, Hatakeyama Y, Ishikawa H, Sawa Y.
  • Organizer: International Association of Dental Research
  • Place of Presentation: Seoul
  • Year and Date: 2016-06-22
  • Int'l Joint Research
• [Presentation] Porphyromonas gingivalis lipopolysaccharide promote diabetic nephropathy through TLR2 (2016)
  • Author(s): Kajiwara K, Takata S, Hatakeyama Y, Ishikawa H, Sawa Y.
  • Organizer: International Association of Dental Research
  • Place of Presentation: Seoul
  • Year and Date: 2016-06-22
  • Int'l Joint Research