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The Development of Novel Protein Intracellular Affinity Pairs for Fabrication of Designer Metabolons.

Research Project

Project/Area Number 15H06859
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Biofunction/Bioprocess
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Mori Yutaro  国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員 (50758539)

Project Period (FY) 2015-08-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsProtein Design / バイオマス / タンパク質工学 / Enzyme Engineering / Metabolon / Self-assembly / Bioproduction / Cell factory
Outline of Final Research Achievements

By assembling metabolic enzymes that continuously perform reactions inside E. coli, this study aims to construct a system that improves efficiency of production of valuable chemicals being targeted. As a preliminary step toward that, the applicant developed and designed new protein affinity pairs that exhibit binding affinity within E. coli cells. In this study we achieved the protein expression of affinity pairs in E. coli, and we confirmed that the protein pairs shows the binding affinity in vitro assay.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report

URL: 

Published: 2015-08-26   Modified: 2018-03-22  

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