食品機能性成分の効率的利用のためのナノ／マイクロキャリアの作製

• Project/Area Number: 15J00406
• Research Category: Grant-in-Aid for JSPS Fellows
• Allocation Type: Single-year Grants
• Section: 国内
• Research Field: Food science
• Research Institution: University of Tsukuba
• Principal Investigator: OSSAI EMMANUEL 筑波大学, 生命環境科学研究科, 特別研究員(DC2)
• Project Period (FY): 2015-04-24 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥1,700,000 (Direct Cost: ¥1,700,000); Fiscal Year 2016: ¥800,000 (Direct Cost: ¥800,000); Fiscal Year 2015: ¥900,000 (Direct Cost: ¥900,000)
• Keywords: Multiple emulsion / Microchannel / Lipid vesicles / Entrapment yield / Carriers / 5-fluorouracil / Metformin hydrochloride / Vesicle stability / Calcein / Reversed-micelles / Bilayer solubilization / Droplet instability

Outline of Annual Research Achievements

In this year, the mechanisms of calcein leakage established in the previous year equally influenced the entrapment yields of 5-fluorouracil (5-FU) and metformin hydrochloride (MH), the antineoplatic and antihyperglycemic agents, respectively. For Tween 80 stabilized vesicle suspension, the entrapment yields of 5-FU and MH increased with decrease in the surfactant concentration, and then decreased at lower surfactant concentration. This trend was attributed to the combined effects of bilayer destabilization/solubilization and reversed-micellar transport. The entrapment yields of the drugs were found to be higher than those reported in the literature. However, the entrapment yield of calcein was remarkably higher than those of the drugs. The difference was explained in relation to their differing molecular weights and relative hydrophobicity. The vesicle suspension was equally found to be stable for about one month of storage at 4 °C. During storage, the entrapment yield decreased slightly and was considered to be due to minimal leakage of the drugs from the internal water phase of the vesicle to the continuous phase through the lipid bilayers. From these studies, the multiple emulsion and solvent evaporation integrated method offers great potentials for the formulation of lipid vesicles with controlled size and high entrapment yields of hydrophilic bioactive molecules, and would find industrial applications in pharmaceuticals, cosmetics, food, and other related fields.

Research Progress Status

28年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

28年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Preparation of lipid vesicles having suitable size for drug delivery with high entrapment of hydrophilic molecules using multiple emulsions (2016)
  • Author(s): Ossai, E.C., Kuroiwa, T., Otsuka, Y., Motokui, Y., Wada, T., Isoda, T., Sato, S. and Ichikawa, S.
  • Journal Title: Journal of Bioengineering & Biomedical Science Volume: 7 (213) Issue: 01 Pages: 1-5
  • DOI: 10.4172/2155-9538.1000213
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Mechanism of surfactant effect on the vesicle preparation using multiple emulsions (2016)
  • Author(s): Ossai, E.C., Terasawa, J., Kanazawa, A., Kuroiwa, T., Sato, S. and Ichikawa, S.
  • Organizer: 17th Annual Meeting, Japan Society for Food Engineering
  • Place of Presentation: Tokyo University of Marine Science and Technology, Shinagawa, Tokyo
  • Year and Date: 2016-08-04
• [Presentation] Efficient entrapment of hydrophilic molecule in lipid vesicles prepared by multiple emulsion method: Effect of surfactant type on the entrapment yield (2016)
  • Author(s): Ossai, E.C., Terasawa, J., Horikoshi, K., Kuroiwa, T., Kanazawa, A., Sato, and S. Ichikawa, S.
  • Organizer: The 10th Conference of Aseanian Membrane Society (AMS 10)
  • Place of Presentation: Nara Kasugano International Forum IRAKA, Nara, Japan.
  • Year and Date: 2016-07-26
  • Int'l Joint Research
• [Presentation] Efficient entrapment of water-soluble molecule in lipid vesicles prepared by multiple emulsion method: Consideration on the mechanism of surfactant effect on the entrapment yield (2016)
  • Author(s): Ossai, E.C., Terasawa, J., Kanazawa, A., Kuroiwa, T. and Ichikawa, S.
  • Organizer: The Society of Separation Process Engineers Japan
  • Place of Presentation: Nihon University, Funabashi, Chiba, Japan.
  • Year and Date: 2016-05-27
• [Presentation] Surfactant type affects the entrapment yield of hydrophilic molecule in lipid vesicles by multiple emulsion method. (2016)
  • Author(s): Ossai, E.C., Terasawa, J., Horikoshi, K., Kuroiwa, T., Kanazawa, A., Sato, S. and Ichikawa, S.
  • Organizer: Tsukuba Students Research Exchange Workshop.
  • Place of Presentation: University of Tsukuba, Tsukuba, Ibaraki.
  • Year and Date: 2016-03-02
• [Presentation] High efficient entrapment of physiologically active substances in lipid vesicles as carriers using multiple emulsion method (2015)
  • Author(s): Ossai, E.C., Terasawa, J., Horikoshi, K., Kuroiwa, T., Kanazawa, A., Sato, S. and Ichikawa, S.
  • Organizer: The University of Tsukuba-National Taiwan University Joint Mini Symposium.
  • Place of Presentation: National Taiwan University, Taipei, ROC
  • Year and Date: 2015-12-08
  • Int'l Joint Research
• [Presentation] Effects of surfactants on the entrapment yield in lipid vesicles by multiple emulsion method. (2015)
  • Author(s): Ossai, E.C., Terasawa, J., Horikoshi, K., Kuroiwa, T., Kanazawa, A., Sato, S. and Ichikawa, S.
  • Organizer: The 47th Autumn Meeting, Society of Chemical Engineers.
  • Place of Presentation: Hokkaido University, Sapporo, Hokkaido.
  • Year and Date: 2015-09-09
• [Presentation] Preparation of size-controlled lipid vesicles entrapping hydrophilic molecules with high efficiency using multiple emulsions. (2015)
  • Author(s): Ossai, E.C., Terasawa, J., Horikoshi, K., Kuroiwa, T., Kanazawa, A., Sato, S. and Ichikawa, S.
  • Organizer: 12th International Congress on Engineering and Food.
  • Place of Presentation: Convention Centre, Quebec City, Canada.
  • Year and Date: 2015-06-14
  • Int'l Joint Research
• [Remarks] Journal of Bioengineering & Biomedical Science
  • URL: https://www.omicsonline.org/open-access/preparation-of-lipid-vesicles-having-suitable-size-for-drug-delivery-withhigh-entrapment-of-hydrophilic-molecules-using-multiple-e-2155-9538-1000213.pdf