ベクターコントロールによる有機塩素系農薬汚染が家畜・ヒトに及ぼす毒性影響の解明

• Project/Area Number: 15J02013
• Research Category: Grant-in-Aid for JSPS Fellows
• Allocation Type: Single-year Grants
• Section: 国内
• Research Field: Environmental agriculture(including landscape science)
• Research Institution: Hokkaido University
• Principal Investigator: Thompson Lesa (2017) 北海道大学, 獣医学研究科, 特別研究員(DC1); THOMPSON LESA (2015-2016) 北海道大学, 獣医学研究科, 特別研究員(DC1)
• Project Period (FY): 2015-04-24 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 2017: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2016: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2015: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: DDT / Chicken / Transcriptomics / Metabolomics / MCF-7 cell culture / Free-range chicken / Human health risk / Microarray / KwaZulu-Natal / Free-ranging chickens / Indoor residual spraying / South Africa

Outline of Annual Research Achievements

Two manuscripts have been published. Various alterations in mRNA expression were detected in chickens exposed to DDTs in the field, including down-regulation of several avian beta-defensins. A manuscript on these findings is currently at the revision stage of publication.mRNA expression was assessed using qRT-PCR after in vitro exposure of p,p’-DDT in MCF-7 human breast cancer cells. Several genes relating to oxidative stress markers, xenobiotic metabolizing enzymes and transporters were significantly up-regulated after exposure. A manuscript has been submitted for peer-review.Samples from in vivo exposure of chickens to p,p’-DDT underwent further analysis. Liver histopathology did not show significant changes. qRT-PCR of liver samples confirmed altered gene expression in avian beta defensin (AvBD) genes, AvBD1 and AvBD7. Further, metabolomics analysis using hydrophilic interaction liquid chromatography (HILIC) revealed many significantly elevated metabolites relating to amino acid metabolism in exposed groups. These results are currently being prepared for journal submission.

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] North West University/BioAssets Consultants/University of Johannesburg(South Africa)
• [Int'l Joint Research] Zagazig University(Egypt)
• [Int'l Joint Research] University of Gondar(Ethiopia)
• [Int'l Joint Research] University of Edinburgh/University of Glasgow/Chester Zoo(英国)
• [Int'l Joint Research] University of Johannesburg/North West University/BioAssets Consultants(South Africa)
• [Int'l Joint Research] Zagazig University(Egypt)
• [Int'l Joint Research] University of Gondar(Ethiopia)
• [Int'l Joint Research] University of Johannesburg/North West University/BioAssets Consultants(South Africa)
• [Int'l Joint Research] Zagazig University(Egypt)
• [Journal Article] Human Health Risk from Consumption of Marine Fish Contaminated with DDT and Its Metabolites in Maputo Bay, Mozambique (2018)
  • Author(s): Thompson L. A.、Ikenaka Y.、Yohannes Y. B.、Ichise T.、Ito G.、Bortey-Sam N.、van Vuren J. J.、Wepener V.、Smit N. J.、Darwish W. S.、Nakayama S. M. M.、Mizukawa H.、Ishizuka M.
  • Journal Title: Bulletin of Environmental Contamination and Toxicology Volume: 100 Issue: 5 Pages: 672-676
  • DOI: 10.1007/s00128-018-2323-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Concentrations and human health risk assessment of DDT and its metabolites in free-range and commercial chicken products from KwaZulu-Natal, South Africa (2017)
  • Author(s): Thompson L. A.、Ikenaka Y.、Yohannes Y. B.、van Vuren J. J.、Wepener V.、Smit N. J.、Darwish W. S.、Nakayama S. M. M.、Mizukawa H.、Ishizuka M.
  • Journal Title: Food Additives and Contaminants: Part A Volume: 34 Issue: 11 Pages: 1959-1969
  • DOI: 10.1080/19440049.2017.1357209
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] β-carotene and retinol reduce benzo[a]pyrene-induced mutagenicity and oxidative stress via transcriptional modulation of xenobiotic metabolizing enzymes in human HepG2 cell line (2017)
  • Author(s): Darwish Wageh Sobhy、Ikenaka Yoshinori、Nakayama Shouta、Mizukawa Hazuki、Thompson Lesa A、Ishizuka Mayumi
  • Journal Title: Environmental Science and Pollution Research Volume: 25 Issue: 7 Pages: 6320-6328
  • DOI: 10.1007/s11356-017-0977-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Organochlorine pesticide contamination of foods in Africa: incidence and public health significance (2017)
  • Author(s): L. A. Thompson, W. S. Darwish, Y. Ikenaka, S. M. Nakayama, H. Mizukawa and M. Ishizuka
  • Journal Title: Journal of Veterinary Medical Science Volume: 79 Issue: 4 Pages: 751-764
  • DOI: 10.1292/jvms.16-0214
  • NAID: 130005568160
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Vector-control pesticide DDT detected in free-ranging chickens from South Africa (2015)
  • Author(s): Lesa A Thompson, Yoshinori Ikenaka, Yared B Yohannes, Johan van Vuren, Victor Wepener, Nico J Smit, Wynand Vlok, Shouta M M Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
  • Journal Title: Proceedings: 7th International Toxicology Symposium in Africa Volume: 7 Pages: 4-5
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] First, do no harm: balancing the benefits of malaria control against the toxic effects of DDT use (2017)
  • Author(s): Thompson, L.A., Ikenaka, Y., Yohannes, Y., Assefa, A., van Vuren, J., Wepener, V., Smit, N., Vlok, W., Darwish, W.S., Nakayama, S., Mizukawa, H., Ishizuka, M.
  • Organizer: 5th Sapporo Summer Seminar for One Health
  • Int'l Joint Research
• [Presentation] Comparison of food products contaminated by DDTs in South Africa and Mozambique (2017)
  • Author(s): Thompson, L.A., Ikenaka, Y., Wepener, V., Ishizuka, M.
  • Organizer: 19th International Conference on One Health
  • Int'l Joint Research
• [Presentation] Effect of DDTs on gallinacins in the avian immune system. (2017)
  • Author(s): Thompson, L.A., Ikenaka, Y., Yohannes, Y., van Vuren, J., Wepener, V., Smit, N., Assefa, A., Darwish, W.S., Nakayama, S., Mizukawa, H., Ishizuka, M.
  • Organizer: The 10th International Meeting of the Asian Society of Conservation Medicine One Health in Asia Pacific
  • Int'l Joint Research
• [Presentation] Genetic effects of the pesticide DDT on birds: lessons learned from a field study in South Africa. (2016)
  • Author(s): Thompson, L.A., Ikenaka, Y., Yohannes, Y., Tsubota, T., van Vuren, J., Wepener, V., Smit, N., Nakayama, S., Mizukawa, H., Ishizuka, M.
  • Organizer: The 9th International Meeting of the Asian Society of Conservation Medicine One Health in Asia Pacific
  • Place of Presentation: Taipei Zoo, Taiwan
  • Year and Date: 2016-10-21
  • Int'l Joint Research
• [Presentation] Playing “chicken” - a xenobiotic dilemma. (2016)
  • Author(s): Thompson, L.A., Ikenaka, Y., Yohannes, Y., Assefa, A., van Vuren, J., Wepener, V., Smit, N., Vlok, W., Nakayama, S.M.M., Mizukawa, H., Ishizuka, M.
  • Organizer: 4th Sapporo Summer Seminar for One Health
  • Place of Presentation: Hokkaido University, Sapporo, Hokkaido, Japan
  • Year and Date: 2016-09-20
  • Int'l Joint Research
• [Presentation] Contamination of eggs from free-ranging chickens in a malaria-endemic region using DDT as vector control. (2016)
  • Author(s): Thompson, L.A., Ikenaka, Y., Yohannes, Y.B., van Vuren, J.J., Wepener, V., Smit, N.J., Vlok, W., Nakayama, S.M.M., Mizukawa, H., Ishizuka, M.
  • Organizer: ISEE-ISES Asia Chapter
  • Place of Presentation: Hokkaido University, Sapporo, Hokkaido, Japan
  • Year and Date: 2016-06-27
  • Int'l Joint Research
• [Presentation] Risks versus benefits of controlling vector-borne diseases using insecticides: a case example in South Africa. (2016)
  • Author(s): Thompson, L.A., Ikenaka, Y., Yohannes, Y.B., van Vuren, J.J., Wepener, V., Smit, N.J., Vlok, W., Nakayama, S.M.M., Mizukawa, H., Ishizuka, M.
  • Organizer: 2nd International Conference on One Medicine One Science
  • Place of Presentation: The Commons Hotel, Minneapolis, USA
  • Year and Date: 2016-04-24
  • Int'l Joint Research
• [Presentation] Vector-contol pesticide DDT detected in free-ranging chickens from South Africa (2015)
  • Author(s): Lesa A THOMPSON, Yoshinori IKENAKA, Yared B YOHANNES, Johan J VAN VUREN, Victor WEPENER, Nico J SMIT, Wynand VLOK, Shouta M M NAKAYAMA, Hazuki MIZUKAWA, Mayumi ISHIZUKA
  • Organizer: 3rd Sapporo Summer Seminar for One Health
  • Place of Presentation: Hokkaido University, Sapporo, Hokkaido, Japan
  • Year and Date: 2015-09-16
  • Int'l Joint Research
• [Presentation] Vector-control pesticide DDT detected in free-ranging chickens from South Africa (2015)
  • Author(s): Lesa A Thompson, Yoshinori Ikenaka, Yared B Yohannes, Johan van Vuren, Victor Wepener, Nico J Smit, Wynand Vlok, Shouta M M Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
  • Organizer: 7th International Toxicology Symposium in Africa
  • Place of Presentation: Garden Court Hotel OR Tambo, Johannesburg, South Africa
  • Year and Date: 2015-08-31
  • Int'l Joint Research / Invited
• [Presentation] Vector-control pesticides disseminating via international waterways to accumulate in free-ranging freshwater and marine fish species (2015)
  • Author(s): Lesa A Thompson, Yoshinori Ikenaka, Yared B Yohannes, Johan van Vuren, Victor Wepener, Nico J Smit, Wynand Vlok, Shouta M M Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
  • Organizer: Vth International Wildlife Management Congress
  • Place of Presentation: Sapporo Convention Center, Sapporo, Hokkaido, Japan
  • Year and Date: 2015-07-26
  • Int'l Joint Research
• [Presentation] Vector-control pesticide DDT detected in free-ranging chickens from South Africa (2015)
  • Author(s): Lesa A Thompson, Yoshinori Ikenaka, Yared B Yohannes, Johan van Vuren, Victor Wepener, Nico J Smit, Wynand Vlok, Shouta M M Nakayama, Hazuki Mizukawa and Mayumi Ishizuka
  • Organizer: 24th Symposium on Environmental Chemistry
  • Place of Presentation: Sapporo Convention Center, Sapporo, Hokkaido, Japan
  • Year and Date: 2015-06-24
• [Remarks] Vetmed Tox Hokkaido University
  • URL: http://tox.vetmed.hokudai.ac.jp/en/
• [Remarks] Vetmed Tox Hokkaido University
• [Remarks] http://tox.vetmed.hokudai.ac.jp/en/
• [Funded Workshop] 4th Sapporo Summer Seminar for One Health, committee chairperson for student session ”Collaboration in One Health: what’s your role?” (2016)
  • Place of Presentation: Hokkaido University, Sapporo, Hokkaido, Japan
  • Year and Date: 2016-09-20
• [Funded Workshop] South Africa - Japan bipartite research symposium, invited presenter, “Extraneous DDT residues in free-ranging chicken products from KwaZulu-Natal, South Africa” (2016)
  • Place of Presentation: Hokkaido University, Sapporo, Hokkaido, Japan
• [Funded Workshop] IWMC, crayfish eradication program (2015)
  • Place of Presentation: Toyako-cho
  • Year and Date: 2015-07-31