| Project/Area Number |
15J05033
|
|---|
| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
|---|
| Section | 国内 |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
宋 紀瑶 名古屋大学, 理学研究科, 特別研究員(DC2)
|
|---|
| Project Period (FY) |
2015-04-24 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2016: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2015: ¥900,000 (Direct Cost: ¥900,000)
|
|---|
| Keywords | Tom40 / mitochondria / yeast / Tom40 channel |
|---|
| Outline of Annual Research Achievements |
Whether the Tom40 channel, which comprise of β-barrel, can be opened laterally has been a hot topic for the past decades. We provided proofs for this possibility. In vitro, we observed fusion proteins with cytochrome b2 holding Om45 pathway targeting signal from the IMS, followed by DHFR for preventing the fusion proteins to go through the Om45 pathway, were inserted into the OM and left the Tom40 channel gradually. In vivo, we observed growth recover from yeast expressing fusion proteins with matrix targeted non-stop protein holding the targeting signal of Om45 pathway, which suggests that non-stop fusion protein does not occupy the Tom40 channel. Proving the possibility of the lateral gating of the Tom40 channel has great impact on understanding the import of mitochondrial proteins.
|
| Research Progress Status |
28年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
28年度が最終年度であるため、記入しない。
|
Report
(2 results)
Research Products
(5 results)
