遺伝子発現に対するＲＮＡ分解および転写後制御の寄与

• Project/Area Number: 15J05075
• Research Category: Grant-in-Aid for JSPS Fellows
• Allocation Type: Single-year Grants
• Section: 国内
• Research Field: Genome biology
• Research Institution: The University of Tokyo
• Principal Investigator: 前川 翔 東京大学, 新領域創成科学研究科, 特別研究員(DC1)
• Project Period (FY): 2015-04-24 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,800,000 (Direct Cost: ¥2,800,000); Fiscal Year 2017: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2016: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2015: ¥1,000,000 (Direct Cost: ¥1,000,000)
• Keywords: RNA分解 / 転写制御 / ゲノム制御 / 次世代シークエンス / トランスクリプトーム制御 / RNA / 次世代シークエンサー

Outline of Annual Research Achievements

The original proposal was to identify the potential role of RNA decay in mediating changes of the eventual RNA abundance.

In the last fiscal year, I was to further refine the RNA decay profile of BRIC seq, further classified the interesting set of genes that were able to be up-regulated via RNA decay, and genes that receive positive feedback and they tend to be transcription factors. I started to conduct validations to confirm that the RNA decay is the main factor that drive changes in gene expression.
In addition I have overlaid the transcription factor data to elucidate the possibility of feedback for the transcription factors.
In addition, I have compared the HIF-1 ChIP seq data to find that HIF-1 and RNA decay seems to be distinct in regulation. Alongside the lab experiments, I have been synthesizing the findings to publish a paper in an academic journal.

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Research Products

• [Presentation] Contribution of RNA decay in mediating changes in the transcriptome in cellular stress (2017)
  • Author(s): Sho Maekawa, Sumio Sugano, Nobuyoshi Akimitsu, Yutaka Suzuki
  • Organizer: The Biology of Genomes
  • Int'l Joint Research
• [Presentation] Regulation of the transcriptome through RNA stability under hypoxia in human colorectal cancer cells (2016)
  • Author(s): Sho Maekawa, Sumio Sugano, Nobuyoshi Akimitsu, Yutaka Suzuki
  • Organizer: The Biology of Genomes
  • Place of Presentation: Cold Spring Harbor, New York, USA
  • Year and Date: 2016-05-10
  • Int'l Joint Research
• [Presentation] Regulation of the transcritome though RNA decay under hypoxia in human colorectal cancer cells (2015)
  • Author(s): Maekawa, S., Sugano, S. Akimitsu, N. and Suzuki, Y
  • Organizer: 分子生物学会年会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Regulation of the transcritome though RNA decay under hypoxia in human colorectal cancer cells, (2015)
  • Author(s): Maekawa, S., Akimitsu, N. and Suzuki, Y
  • Organizer: RNA学会年会
  • Place of Presentation: ホテルライフォート（北海道札幌市）
  • Year and Date: 2015-07-14
• [Presentation] RNA decay factor mediated RNA stability contributions on RNA abundanc (2015)
  • Author(s): Maekawa, S., Imamachi, N., Irie, T., Tani, H., Matsumoto, K., Mizutani, R., Imamura, K., Kakeda, M., Yada, T., Sugano, S., Suzuki, Y. and Akimitsu, N.
  • Organizer: The 11th International Workshop on Advanced Genomics
  • Place of Presentation: 学術総合センター一橋講堂（東京都千代田区）
  • Year and Date: 2015-05-20
  • Int'l Joint Research
• [Presentation] RNA decay factor mediated RNA stability contributions on RNA abundance (2015)
  • Author(s): Maekawa, S., Imamachi, N., Irie, T., Tani, H., Matsumoto, K., Mizutani, R., Imamura, K., Kakeda, M., Yada, T., Sugano, S., Suzuki, Y. and Akimitsu, N.
  • Organizer: Biology of Genomes
  • Place of Presentation: Cold Spring Habor, NY, USA
  • Year and Date: 2015-05-06
  • Int'l Joint Research
• [Book] RNA-seqマスターブック (2016)
  • Author(s): 前川翔、鈴木絢子
  • Publisher: 羊土社