イメージングと時系列統計解析による細胞死制御機構の定量的な解析
| Project/Area Number |
15J08196
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|---|
| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
|---|
| Section | 国内 |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | Kyoto University |
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Principal Investigator |
三浦 晴子 京都大学, 生命科学研究科, 特別研究員(DC1)
|
|---|
| Project Period (FY) |
2015-04-24 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2017: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2016: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2015: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | p38 / JNK / cell death / crosstalk / live cell imaging / single cell analysis |
|---|
| Outline of Annual Research Achievements |
I successfully applied a live cell imaging system to visualize the activities of the stress-activated kinases JNK and p38 simultaneously in single cells. Using this system, I discovered cell-to-cell variability of JNK activity in response to various stresses. I found regulatory mechanisms involving p38 kinase which generated the heterogeneity in JNK activity. My data indicate that the heterogeneity in JNK activity determines cell fate decisions in response to severe stresses, such as UV. Statistical analysis of p38 and JNK activity dynamics identified parameters which were predictive for cell death. The work was summarized in a paper and submitted to a peer-reviewed journal. In summary, I made good progress according to the initial plans.
|
| Research Progress Status |
29年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
29年度が最終年度であるため、記入しない。
|
Report
(3 results)
Research Products
(13 results)
