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Development of ultra-high-amylose rice starch of BEs triple mutant and study of its properties for utilization

Research Project

Project/Area Number 15K00779
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Eating habits
Research InstitutionNiigata University

Principal Investigator

Itoh Kimiko  新潟大学, 自然科学系, 教授 (10281007)

Co-Investigator(Kenkyū-buntansha) 花城 勲  鹿児島大学, 農水産獣医学域農学系, 准教授 (30336325)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsBranchin Enzyme / Starch Synthase / BE / rice / starch / GBSSI / amylose / amylopectin / コメ / デンプン / 超高アミロース / Branching Enzyme / デンプン粒 / Wha / 高アミロース / デンプン枝作り酵素
Outline of Final Research Achievements

To develop starch with a novel property, a ΔBEs/Wxa line with the reduced expression of three starch branching enzymes was developed. The appearance of trait of the mature seeds was white and opaque, and floury. Round and small starch grain was observed in the cross section of the seeds. The X-ray diffraction pattern of the ΔBEs/Wxa starch showed B type pattern. The unit chain length distribution of the starch showed that short- and long- chain fractions were greatly reduced. The apparent amylose content was up to 65% maximum, calculated from weight distribution of the unit chains. The true amylose content was up to 47.7 % maximum. Since the ΔBEs/Wxa starch was fine grain, round, and floury, it is expected to be used as an ultrafine grain powder.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (11 results)

All 2018 2016 2015 Other

All Int'l Joint Research (2 results) Presentation (8 results) (of which Invited: 1 results) Remarks (1 results)

  • [Int'l Joint Research] Public University of Navarra/IAB, CSIC(SPAIN)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] Public University of Navarra(Spain)

    • Related Report
      2015 Research-status Report
  • [Presentation] BE発現抑制による超高アミロース・コメデンプンの開発2018

    • Author(s)
      後藤大地、阿部克、花城勲、白鳥龍一、中村保典、伊藤紀美子
    • Organizer
      日本農芸化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Whole-genome sequencing reveals a large deletion on chromosome 6 in a rice wx mutant.2016

    • Author(s)
      Nao Nomura, Takeshi Takamatsu, Hidekazu Shimizu, Toshiaki Mitsui, Kimiko Itoh
    • Organizer
      KAAB International Symposium 2016
    • Place of Presentation
      Niigata University(Niigata City・Niigata)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] Comparative analysis of endosperm proteomes among seven rice cultivars vary in physicochemical properties of the starches.2016

    • Author(s)
      Ami Hyono, Mari Wtanabe, Masataka Nihei, Kentaro Kaneko, Toshiaki Mitsui, Kimiko Itoh
    • Organizer
      KAAB International Symposium 2016
    • Place of Presentation
      Niigata University(Niigata City・Niigata)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] Production and characterization of novel glycogen- based polymer2015

    • Author(s)
      Mamiko FUKUSHIMA, Goizeder ALMAGRO, Isao HANASHIRO, Javier POZUETA ROMERO, Toshiaki MITSUI, Kimiko ITOH
    • Organizer
      KAAB International Symposium 2015
    • Place of Presentation
      Niigata University, Niigata City
    • Year and Date
      2015-09-29
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Study of in vitro ELC(Extra -long chain) synthesis by rice GBSSI2015

    • Author(s)
      Ami HYONO, Mina YAMAZAKI, Michiyo TAKAHASHI, Kentaro KANEKO, Kimiko ITOH
    • Organizer
      KAAB International Symposium 2015
    • Place of Presentation
      Niigata University, Niigata City
    • Year and Date
      2015-09-29
    • Related Report
      2015 Research-status Report
  • [Presentation] Comparative proteomics of rice endosperm proteins from seven cultivars, differences in physicochemical properties of the starches.2015

    • Author(s)
      Masataka NIHEI, Mari WATANABE, Kentaro KANEKO, Kimiko ITOH, Toshiaki MITSUI
    • Organizer
      KAAB International Symposium 2015
    • Place of Presentation
      Niigata University, Niigata City
    • Year and Date
      2015-09-29
    • Related Report
      2015 Research-status Report
  • [Presentation] 質量分析器を用いた澱粉物性の異なるイネ品種間のプ ロテオーム解析2015

    • Author(s)
      伊 藤 紀美子・渡 邊 茉 莉・金 古 堅太郎・ 二 瓶 正 崇・三ツ井 敏 明
    • Organizer
      日本応用糖質科学 会平成28年度大会 (第65回)・応用糖 質科学シンポジウ ム
    • Place of Presentation
      奈良春日野国際フォーラム, 奈良市
    • Year and Date
      2015-09-16
    • Related Report
      2015 Research-status Report
  • [Presentation] 澱粉物性の異なるイネ品種の胚乳における澱粉合成関 連タンパク質群の解析2015

    • Author(s)
      二 瓶 正 崇・渡 邊 茉 莉・金 古 堅太郎・ 伊 藤 紀美子・三ツ井 敏 明
    • Organizer
      第56回新潟生化学 懇話会/平成27年 度日本生化学会関 東支部例会
    • Place of Presentation
      メディアシップ, 新潟市
    • Year and Date
      2015-06-20
    • Related Report
      2015 Research-status Report
  • [Remarks] 新潟大学農学部 教員紹介

    • URL

      http://www.agr.niigata-u.ac.jp/profile/itou/index.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-05-15  

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