| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
In order to prepare transplantable hepatocyte sheets with maintaining hepatic functions, a thermoresponsive culture system mimicking biological environments was designed. Transplantable hepatocyte sheets could be prepared while maintaining hepatic functions on a heparin-immobilized thermoresponsive surface containing a component of basement membrane such as growth factors. It was also possible to quantitatively evaluate the temperature-dependent affinity between cellular receptor/growth factor/the heparin-immobilized thermoresponsive surfaces. By using flexible basal substrate and microfluidic devices, more advanced hepatocyte sheet tissue culture system would be constructed.
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