The cutaneous penetration studies of the human stratum corneum under an electric and ultrasound field using a new method.

• Project/Area Number: 15K05253
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological physics/Chemical physics/Soft matter physics
• Research Institution: Kwansei Gakuin University
• Principal Investigator: Nakazawa Hiromitsu 関西学院大学, 理工学部, 教育技術主事 (70411775)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 経皮吸収 / 角層 / 細胞間脂質 / X線構造解析 / ソフトマター / バリア機能 / TEWL / 皮膚角層 / 医薬品 / イオントフォレシス / 化粧品 / 経皮吸収促進 / X線回折

Outline of Final Research Achievements

Stratum corneum (SC), the outermost layer of the human skin, plays a crucial role for the skin barrier. Because the SC also serves as a physicochemical barrier to percutaneous absorption of drugs, so in order to develop drugs with more effective absorption properties, it is important to clarify the interaction between the SC and drugs at the molecular level. In this study, by using the sample holder designed for the simultaneous analysis of the SC structure and skin permeation that the author developed alone, the behavior of water molecules in SC and the effect of external treatment such as electric filed on the skin permeability were clarified.

Academic Significance and Societal Importance of the Research Achievements

皮膚疾患に対する外皮用薬（塗り薬）やスキンケアなどの分野における薬剤、経皮吸収促進剤等の開発分野においては、薬剤と角層との相互作用や、それに対する非侵襲的な外部刺激の影響に関する情報が強く求められている。本研究により、実用的な経皮吸収性評価手法が確立され、角層の構造と機能の関係が明らかになれば、吸収性に優れた外皮用薬や化粧品の効率的な研究開発が期待される。また、アトピー性皮膚炎等の病変皮膚における構造変化やアレルゲンの吸収特性の解明にも寄与できる。

Research Products

• [Journal Article] Simultaneous Measurements of Structure and Water Permeability in an Isolated Human Skin Stratum Corneum Sheet (2019)
  • Author(s): Hiromitsu Nakazawa, Tomohiro Imai, Mika Suzuki, Natsuki Akakabe, Ichiro Hatta, Satoru Kato
  • Journal Title: Polymers Volume: 11 Pages: 829
  • DOI: 10.3390/polym11050829
  • Peer Reviewed / Open Access
• [Journal Article] A Possible Percutaneous Penetration Pathway That Should Be Considered. (2017)
  • Author(s): I. Hatta, N. Ohta, H. Nakazawa
  • Journal Title: Pharmaceutics. Volume: 9 Pages: 26-34
  • DOI: 10.3390/pharmaceutics9030026.
  • Peer Reviewed / Open Access
• [Journal Article] ヒト皮膚角層の構造と物質透過性 (2015)
  • Author(s): 加藤 知、中沢 寛光
  • Journal Title: オレオサイエンス Volume: 15(11) Pages: 503-510
  • Acknowledgement Compliant
• [Presentation] ヒト角層モデル膜の開発と経皮吸収試験への応用 (2018)
  • Author(s): 中沢寛光、赤下部奈月、加藤知
  • Organizer: SPring-8フロンティアソフトマター開発専用ビームライン第8回研究発表会
• [Presentation] Development and analysis of model lipid membrane designed for the examination of skin barrier. (2018)
  • Author(s): N. Akakabe, H. Nakazawa
  • Organizer: The 30th Conference of the European Colloid and Interface Society
  • Int'l Joint Research
• [Presentation] 外部環境制御セルを用いたヒト皮膚角層の物質透過性の解析 (2017)
  • Author(s): 赤下部 奈月、中沢 寛光、内田 崇志、佐野友彦
  • Organizer: 第7回化粧品開発展アカデミックフォーラム
  • Place of Presentation: 東北大学（宮城県・仙台市）
  • Year and Date: 2017-03-24 – 2017-03-27
• [Presentation] 外部環境制御セルを用いたヒト皮膚角層の物質透過性の解析 (2017)
  • Author(s): 中沢 寛光
  • Organizer: 第7回化粧品開発展アカデミックフォーラム
  • Place of Presentation: 東京国際展示場（東京都・江東区）
  • Year and Date: 2017-01-23 – 2017-01-25
• [Presentation] Development of model lipid membranes based on non-hydroxy phytosphingosine for the studies of skin barrier properties. (2017)
  • Author(s): N. Akakabe, H. Nakazawa, et al.
  • Organizer: The 29th Conference of the European Colloid and Interface Society
  • Int'l Joint Research
• [Presentation] 角層細胞間脂質モデル膜を用いたセラミド分子種の特性解析. (2017)
  • Author(s): 赤下部 奈月, 中沢 寛光
  • Organizer: 日本薬学会第137年回 (仙台)
• [Presentation] 外部環境制御セルを用いたヒト皮膚角層の物質透過性の解析. (2017)
  • Author(s): 中沢 寛光
  • Organizer: 第7回化粧品開発展アカデミックフォーラム (東京)
• [Presentation] The effect of solvent on the hydrogel composed of a hydrogenated lecithin and fatty alcohol. (2016)
  • Author(s): Y. Nakagawa, H. Nakazawa, S. Kato
  • Organizer: The 28th Conference of the European Colloid and Interface Society
  • Place of Presentation: ローマ（イタリア）
  • Year and Date: 2016-09-04 – 2016-09-09
  • Int'l Joint Research
• [Presentation] Effect of external factors on the human skin corneocyte morphology. (2016)
  • Author(s): S. Maeda, H. Nakazawa, S. Kato
  • Organizer: The 28th Conference of the European Colloid and Interface Society
  • Place of Presentation: ローマ（イタリア）
  • Year and Date: 2016-09-04 – 2016-09-09
  • Int'l Joint Research
• [Presentation] Regulation Mechanism of Water Content in Stratum Corneum (2016)
  • Author(s): I. Hatta, H. Nakazawa, N. Ohta
  • Organizer: Skin Forum 2016 Annual Meeting
  • Place of Presentation: ロンドン（イギリス）
  • Year and Date: 2016-06-21 – 2016-06-22
  • Int'l Joint Research
• [Presentation] ヒト角層構造を模倣したモデル人工膜の開発研究 (2016)
  • Author(s): 中沢 寛光・米永 沙世・加藤 知
  • Organizer: フロンティアソフトマター開発専用ビームライン産学連合体 第5回 成果報告会
  • Place of Presentation: 名古屋工業大学（愛知県・名古屋市）
  • Year and Date: 2016-01-18 – 2016-01-19
• [Presentation] 電子線回折法及びＸ線回折法を用いた皮膚角層の構造解析 (2015)
  • Author(s): 中沢寛光
  • Organizer: 第４回 関西ものづくり技術シーズ発表会
  • Place of Presentation: 追手門大学（大阪府・大阪市）
  • Year and Date: 2015-10-01
• [Presentation] 皮膚角層中の水の動的振舞 (2015)
  • Author(s): 八田一郎・中沢寛光・太田昇
  • Organizer: 日本油化学会第54回年会
  • Place of Presentation: 名城大学（愛知県・名古屋市）
  • Year and Date: 2015-09-08 – 2015-09-10
• [Presentation] Development of model lipid membranes to elucidate the role of stratum corneum intercellular lipid lamellar structures in the skin barrier function. (2015)
  • Author(s): S. Yonenaga, H. Nakazawa, T. Uchida, S. Kato
  • Organizer: The 27th Conference of the European Colloid and Interface Society
  • Place of Presentation: ボルドー（フランス）
  • Year and Date: 2015-09-06 – 2015-09-11
  • Int'l Joint Research
• [Presentation] Study on human corneocyte morphology by newly developed non-invasive cryosection electron microscopy. (2015)
  • Author(s): S. Maeda, K. Nakamura, H. Nakazawa, S. Kato
  • Organizer: The 27th Conference of the European Colloid and Interface Society
  • Place of Presentation: ボルドー（フランス）
  • Year and Date: 2015-09-06 – 2015-09-11
  • Int'l Joint Research
• [Patent(Industrial Property Rights)] 敏感肌の評価方法 (2016)
  • Inventor(s): 内田崇志、加藤知、中沢寛光
  • Industrial Property Rights Holder: 内田崇志、加藤知、中沢寛光
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特願2016-045327
  • Filing Date: 2016-03-09