| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In the present research, the combinational use of 1D NMR-based screening techniques with 1H and 19F detections were applied to a protein-ligand complex. The highly sensitive and selective methods to observe ligands at the atomic level were developed. In purpose of identifying the 1H and 19F binding epitopes of the fluorinated compounds, the combinatorial analysis using 1H{1H} and 19F{1H} saturation transfer difference (STD) experiments and were carried out. Because 19F of the fluorinated compounds was detected, the sample solutions containing 95% H2O+5% D2O can be easily applied using the proposed methods.
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