Development of rapid DNA detection method for multiplex virus detection
| Project/Area Number |
15K06111
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Measurement engineering
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| Research Institution | Kyushu University |
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Principal Investigator |
Nakano Michihiko 九州大学, システム情報科学研究院, 准教授 (00447856)
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| Co-Investigator(Renkei-kenkyūsha) |
SUEHIRO Junya 九州大学, 大学院システム情報科学研究院, 准教授 (70206382)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | DNA検出 / 誘電泳動 / インピーダンス計測 / ウイルス検査 / 微粒子 / 高感度検出 / バイオセンサ / インピーダンス / 周波数特性 / PCR / 遺伝子検査 / Multiplex PCR |
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| Outline of Final Research Achievements |
Applications of microbeads dielectrophoresis (DEP) based DNA detection method were investigated. (1) DEP change of microbead by DNA attachment is derived from not only electric charges on DNA but also permittivity of DNA, (2) application on food contamination by detecting pig mitochondrial DNA sensitive , (3) quantitative detection of norovirus gene (10 copies/reaction) in comparing with real-time polymerase chain reaction, and (4) determination of DNA length by measuring frequency dependent conductance change of DNA attached microbeads. These results suggest that the DNA detection can be applied to detect multiple virus target genes.
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Report
(4 results)
Research Products
(38 results)
