| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Understanding the phenomena for habitat isolation of a heterogeneous cell population in a three-dimensional constructs will be useful for developing biomimetic and functional engineered tissues. To examine the habitation of co-cultured cells, a five-layered human skeletal muscle myoblast (HSMM) sheet, regarded as a plate-shaped aggregate, was overlaid onto target cells, namely human skeletal muscle fibroblasts (HSMFs) and human umbilical vein endothelial cells (HUVECs), with different initial densities on the bottom surface to investigate the behaviors of target cells in the plate-shaped aggregate. HSMFs rapidly and linearly migrated in the vertical direction after detaching from the bottom surface; cells reached the upper layers. HUVECs show a horizontally elongated shape to maintain their horizontal connections with neighboring cells; this led to slow migration in the vertical direction through the formation of net-shaped aggregates in the middle of the five-layered sheet.
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